(1)背景:胸膜的早期间皮反应,导致纤维化,已在化学或重金属暴露后的动物中报告。然而,对早期时序间皮反应相关冷冻损伤的视觉监测尚未得到充分研究.因此,本研究旨在评估和观察兔胸膜冷冻损伤后的早期间皮反应。(2)方法:我们使用光学相干断层扫描(OCT)监测了冷冻伤后兔胸膜的早期间皮反应,实时,然后与病理图像进行比较。由于OCT的穿透极限,我们制作了一个胸窗,在体内对顶叶和内脏胸膜进行成像。我们还使用了一种创新的技术来捕获体内的微观结构,采用计算机控制的间歇性等压屏住呼吸以减少呼吸运动,提高OCT的分辨率。我们组织了三个样本组:正常组,只有一个胸窗的假小组,实验组采用胸窗和冷冻治疗。在实验组中,进行局部冷冻损伤。在前30分钟内通过OCT观察冷冻治疗损伤部位胸膜水平处的间皮细胞,然后在2天后在相同部位再次观察。(3)结果:实验组,首次损伤后,使用OCT在冷冻损伤部位观察到壁胸膜局灶性增厚,经病理证实为局灶性间皮细胞增殖。冷冻伤后两天,在同一只兔子的冷冻损伤部位周围,在顶叶胸膜中发现了弥漫性间皮细胞增殖。在假小组中,未发现胸膜反应。OCT和病理检查显示,顶叶和内脏胸膜之间的间皮细胞反应模式不同:在顶叶胸膜中发现了间皮细胞的局灶性增殖,而在内脏胸膜中仅发现了从扁平细胞到立方形细胞的形态变化和增厚的单层,而间皮细胞没有增殖。(4)结论:胸膜和内脏胸膜冷冻损伤后发生早期间皮反应。
(1) Background: An early mesothelial reaction of the pleura, leading to fibrosis, has been reported in animals after chemical or heavy metal exposure. However, the visual monitoring of early time-sequential mesothelial reaction-associated
cryoinjury has not been fully investigated. Therefore, this study aimed to evaluate and visualize the early mesothelial reactions seen following
cryoinjury using rabbit pleura. (2) Methods: We monitored the early mesothelial reaction in rabbit pleurae after
cryoinjury using optical coherence tomography (OCT), in real-time, which was then compared with pathological images. Due to the penetration limit of OCT, we made a thoracic window to image the parietal and visceral pleurae in vivo. We also used an innovative technique for capturing the microstructure in vivo, employing a computer-controlled intermittent iso-pressure breath hold to reduce respiratory motion, increasing the resolution of OCT. We organized three sample groups: the normal group, the sham group with just a thoracic window, and the experimental group with a thoracic window and cryotherapy. In the experimental group, localized
cryoinjury was performed. The mesothelial cells at the level of pleura of the cryotherapy-injured site were visualized by OCT within the first 30 min and then again after 2 days at the same site. (3) Results: In the experimental group, focal thickening of the parietal pleura was observed at the site of cryoinjury using OCT after the first injury, and it was then confirmed pathologically as focal mesothelial cell proliferation. Two days after
cryoinjury, diffuse mesothelial cell proliferation in the parietal pleura was noted on the reverse side around the cryoinjured site in the same rabbit. In the sham group, no pleural reaction was found. The OCT and pathological examinations revealed different patterns of mesothelial cell reactions between the parietal and visceral pleurae: the focal proliferation of mesothelial cells was found in the parietal pleura, while only a morphological change from flat cells to cuboidal cells and a thickened monolayer without proliferation of mesothelial cells were found in the visceral pleural. (4) Conclusions: An early mesothelial reaction occurs following cryoinjury to the parietal and visceral pleurae.