PDF(Pubmed)
Abstract：
UNASSIGNED: The use of extremely low temperatures in vitrification is known to cause cryoinjury so that it can trigger the activation of the intrinsic apoptotic pathway, which can damage the structural integrity of the pre-antral follicle. Based on that, it is necessary to use an appropriate cryoprotectant to protect the preserved cell.
UNASSIGNED: This study aimed to identify the potential use of date juice concentrate (DJC) as a natural extracellular cryoprotectant to suppress the rate of apoptosis after vitrification.
UNASSIGNED: This experimental research uses 24 samples of ovarian rats. Rats were fed and drank an ad libitum.
UNASSIGNED: Ovaries were isolated in the proestrus phase, then processed into slides for immunohistochemistry (IHC) staining using anti-Bax and anti-Bcl-2 antibodies. IHC results were evaluated for the brown colour using ImageJ IHC Profiler. The results were analysed as an optical density and displayed in the Bax/Bcl-2 ratio.
UNASSIGNED: All data were statistically analysed with either parametric (analysis of various) or non-parametric (Kruskal-Wallis) tests.
UNASSIGNED: The combination of EG 7.5% + DJC 15% (KP2) showed the lowest Bax/Bcl-2 ratio in primordial and primary follicles. Meanwhile, the lowest Bax/Bcl-2 ratio in secondary follicles is found in KP4 (EG 15% + DJC 15%). The DJC is known to contain a dominant amount of glucose. The DJC shows antioxidant activity and contains antioxidant compounds, phenols and flavonoids.
UNASSIGNED: The sugar content and antioxidant compounds of DJC can protect against follicle membrane damage, so the rate of intrinsic apoptosis pathway is also suppressed initially with Bax protein suppression in the mitochondrial membrane.
UNASSIGNED: This study aimed to identify the potential use of date juice concentrate (DJC) as a natural extracellular cryoprotectant to suppress the rate of apoptosis after vitrification.
UNASSIGNED: This experimental research uses 24 samples of ovarian rats. Rats were fed and drank an ad libitum.
UNASSIGNED: Ovaries were isolated in the proestrus phase, then processed into slides for immunohistochemistry (IHC) staining using anti-Bax and anti-Bcl-2 antibodies. IHC results were evaluated for the brown colour using ImageJ IHC Profiler. The results were analysed as an optical density and displayed in the Bax/Bcl-2 ratio.
UNASSIGNED: All data were statistically analysed with either parametric (analysis of various) or non-parametric (Kruskal-Wallis) tests.
UNASSIGNED: The combination of EG 7.5% + DJC 15% (KP2) showed the lowest Bax/Bcl-2 ratio in primordial and primary follicles. Meanwhile, the lowest Bax/Bcl-2 ratio in secondary follicles is found in KP4 (EG 15% + DJC 15%). The DJC is known to contain a dominant amount of glucose. The DJC shows antioxidant activity and contains antioxidant compounds, phenols and flavonoids.
UNASSIGNED: The sugar content and antioxidant compounds of DJC can protect against follicle membrane damage, so the rate of intrinsic apoptosis pathway is also suppressed initially with Bax protein suppression in the mitochondrial membrane.
摘要：
■已知在玻璃化中使用极低的温度会引起冷冻损伤，从而可以触发内在凋亡途径的激活，这可能会破坏窦前卵泡的结构完整性。基于此,有必要使用适当的冷冻保护剂来保护保存的细胞。
■本研究旨在确定椰枣汁浓缩物（DJC）作为天然细胞外冷冻保护剂的潜在用途，以抑制玻璃化后的凋亡率。
■这项实验研究使用了24只卵巢大鼠的样本。给大鼠喂食并随意饮用。
■卵巢在发情期分离，然后处理成载玻片用于使用抗Bax和抗Bcl-2抗体的免疫组织化学(IHC)染色。使用ImageJIHC剖面仪评估棕色的IHC结果。结果作为光密度分析并以Bax/Bcl-2比率显示。
用参数(各种分析)或非参数(Kruskal-Wallis)检验对所有数据进行统计学分析。
■EG7.5％DJC15％（KP2）的组合在原始和初级卵泡中显示出最低的Bax/Bcl-2比率。同时,次级卵泡中Bax/Bcl-2比率最低的是KP4(EG15%+DJC15%)。已知DJC含有主要量的葡萄糖。DJC显示抗氧化活性，含有抗氧化化合物，酚类和类黄酮。
■DJC的糖含量和抗氧化化合物可以防止卵泡膜损伤，因此，内源性凋亡途径的速率最初也被线粒体膜中的Bax蛋白抑制所抑制。
■本研究旨在确定椰枣汁浓缩物（DJC）作为天然细胞外冷冻保护剂的潜在用途，以抑制玻璃化后的凋亡率。
■这项实验研究使用了24只卵巢大鼠的样本。给大鼠喂食并随意饮用。
■卵巢在发情期分离，然后处理成载玻片用于使用抗Bax和抗Bcl-2抗体的免疫组织化学(IHC)染色。使用ImageJIHC剖面仪评估棕色的IHC结果。结果作为光密度分析并以Bax/Bcl-2比率显示。
用参数(各种分析)或非参数(Kruskal-Wallis)检验对所有数据进行统计学分析。
■EG7.5％DJC15％（KP2）的组合在原始和初级卵泡中显示出最低的Bax/Bcl-2比率。同时,次级卵泡中Bax/Bcl-2比率最低的是KP4(EG15%+DJC15%)。已知DJC含有主要量的葡萄糖。DJC显示抗氧化活性，含有抗氧化化合物，酚类和类黄酮。
■DJC的糖含量和抗氧化化合物可以防止卵泡膜损伤，因此，内源性凋亡途径的速率最初也被线粒体膜中的Bax蛋白抑制所抑制。
