PDF(Pubmed)
Abstract：
Benign Prostatic Hyperplasia (BPH) is a pathological condition that directly interferes with the reproductive potential of senile dogs, by leading to prostate enlargement and sperm injury, which in turn may compromise sperm freezeability. Moreover, albeit finasteride treatment reduces prostatic volume and blood supply and maintains seminal quality and testicular integrity, the effects of sperm samples submitted to cryopreservation after the finasteride treatment are still unknown. Thus, the aim of this study was to evaluate frozen-thawed semen of BPH dogs, as well as dogs subjected to BPH pharmacological treatment with finasteride. For such purpose, 20 dogs were previously selected and assigned to three experimental groups, according to BPH diagnosis and treatment with finasteride: Control (n = 9), BPH Group (n = 5) and BPH-Finasteride Group (n = 6). Semen was subjected to one-step cryopreservation protocol with tris-fructose-citric acid extender with 5% glycerol and thawed at 37°C for 30 sec. Fresh and post-thaw sperm samples were evaluated for macroscopic parameters, sperm concentration, sperm motility kinetics, sperm mitochondrial activity and potential, oxidative stress, plasmatic and acrosome membrane integrity, sperm DNA fragmentation and sperm binding test on perivitelic membrane of chicken egg yolk. Regarding fresh semen, BPH-Finasteride group had the lowest ejaculate visual aspect (opacity), higher frequency of sperm flagellar beating (BCF) and percentage of sperm with medium velocity. Control group had the highest percentage of sperm DNA integrity compared to BPH group. For the frozen-thawed semen, Control group presented the highest percentage of spermatozoa with high mitochondrial activity. However, the BPH-Finasteride group showed higher number of sperm bound to the perivitelline membrane of chicken egg yolk compared to the BPH Group. Conversely, BPH group had higher percentage of DNA damage. In conclusion, the ejaculate of BPH dogs has higher susceptibility to cryoinjury, whereas finasteride-treated dogs have increased spermatozoa functional performance, suggesting a promising use of BPH dogs as semen donors in sperm cryopreservation programs.
摘要：
良性前列腺增生（BPH）是一种直接干扰老年犬生殖潜能的病理状态，导致前列腺肿大和精子损伤，这反过来可能会损害精子的冷冻能力。此外，尽管非那雄胺治疗减少了前列腺体积和血液供应，并保持了精液质量和睾丸完整性，非那雄胺治疗后接受冷冻保存的精子样本的效果仍然未知.因此，这项研究的目的是评估BPH犬的冻融精液，以及用非那雄胺进行BPH药物治疗的狗。为此,先前选择了20只狗，并将其分配到三个实验组，根据BPH诊断和非那雄胺治疗：对照（n=9），BPH组(n=5)和BPH-非那雄胺组(n=6)。用含5%甘油的tris-果糖-柠檬酸增量剂对精液进行一步冷冻保存方案,并在37°C下解冻30秒。对新鲜和解冻后的精子样品进行了宏观参数评估，精子浓度,精子运动动力学，精子线粒体活动和潜力，氧化应激，血浆和顶体膜的完整性，鸡卵黄卵周膜精子DNA断裂和精子结合试验。关于新鲜精液，BPH-非那雄胺组射精视觉方面（不透明）最低，精子鞭毛跳动（BCF）的频率更高，中等速度的精子百分比更高。与BPH组相比，对照组的精子DNA完整性百分比最高。冻融的精液,对照组的精子比例最高，线粒体活性高。然而,与BPH组相比，BPH-非那雄胺组显示出更多的精子与鸡蛋黄的卵周膜结合。相反，BPH组DNA毁伤百分率较高。总之,BPH犬的射精对冷冻损伤的易感性更高，而非那雄胺治疗的狗精子功能表现增加，建议在精子冷冻保存计划中使用BPH犬作为精液供体。
