水杨酸(SA)作为一种激素在植物中起着至关重要的作用,属于酚类化合物。我们的目标是确定SA的最佳浓度,以增强Agastacherugosa植物中生物活性化合物的产生,同时保持最佳的植物生长。对植物进行了不同浓度的SA浸泡处理(即,0、100、200、400、800和1600μmolmol-1)在移植后7天10分钟。我们观察到800和1600μmol-1的SA水平升高会引起氧化应激,导致许多植物生长变量的显著减少,包括叶子长度,宽度,number,area,射击鲜重(FW),茎FW和长度,和整株植物干重(DW)与对照植物相比。此外,用1600μmolmol-1SA处理导致花枝数的最低值,花的FW和DW,和叶子的DW,茎,和根。相反,施用400μmol-1的SA导致叶绿素(Chl)a和b的最大增加,总Chl,总黄酮,总类胡萝卜素,和SPAD值。光合速率和气孔导度随着SA浓度的增加而降低(即,800和1600μmol-1)。此外,较高的SA处理(即,400、800和1600μmol-1)提高了酚类含量,几乎所有的SA处理都增加了抗氧化能力。迷迭香酸含量在200μmol-1SA处理下达到峰值。然而,在400μmol-1SA下,tilianin和acacetin含量达到最高水平。这些发现表明,将根浸入200和400μmol-1SA中可以增强水培培养的红花中生物活性化合物的产生,而不会损害植物的生长。总的来说,这些发现提供了有关SA对A的影响的有价值的见解。rugosa及其对药用植物种植和植物化学生产的潜在影响。
Salicylic acid (SA) plays a crucial role as a hormone in plants and belongs to the group of phenolic compounds. Our objective was to determine the optimal concentration of SA for enhancing the production of bioactive compounds in Agastache rugosa plants while maintaining optimal plant growth. The plants underwent SA soaking treatments at different concentrations (i.e., 0, 100, 200, 400, 800, and 1600 μmol mol-1) for 10 min at 7 days after they were transplanted. We observed that elevated levels of SA at 800 and 1600 μmol mol-1 induced oxidative stress, leading to a significant reduction across many plant growth variables, including leaf length, width, number, area, shoot fresh weight (FW), stem FW and length, and whole plant dry weights (DW) compared with that in the control plants. Additionally, the treatment with 1600 μmol mol-1 SA resulted in the lowest values of flower branch number, FW and DW of flowers, and DW of leaf, stem, and root. Conversely, applying 400 μmol mol-1 SA resulted in the greatest increase of chlorophyll (Chl) a and b, total Chl, total flavonoid, total carotenoid, and SPAD values. The photosynthetic rate and stomatal conductance decreased with increased SA concentrations (i.e., 800 and 1600 μmol mol-1). Furthermore, the higher SA treatments (i.e., 400, 800, and 1600 μmol mol-1) enhanced the phenolic contents, and almost all SA treatments increased the antioxidant capacity. The rosmarinic acid content peaked under 200 μmol mol-1 SA treatment. However, under 400 μmol mol-1 SA, tilianin and acacetin contents reached their highest levels. These findings demonstrate that immersing the roots in 200 and 400 μmol mol-1 SA enhances the production of bioactive compounds in hydroponically cultivated A. rugosa without compromising plant growth. Overall, these findings provide valuable insights into the impact of SA on A. rugosa and its potential implications for medicinal plant cultivation and phytochemical production.