■丹参被认为是一种药用植物,在伊朗分布广泛。在伊朗的传统医学中,它也用于治疗利什曼病,疟疾,动脉粥样硬化,心血管疾病,作为消毒剂。本研究旨在确定该植物茎中的抗念珠菌成分和抗毛滴虫天然化合物。
■收集植物芽,干,去除叶子后,接地。在浸渍罐中提取干燥的植物材料,由Rotavap集中,脱脂,并通过普通柱层析进行分馏。基于对念珠菌的抗真菌筛选,Fr.4,具有更多的抗真菌活性,被选作植物化学分析,采用不同的色谱方法对硅胶柱和SephadexLH-20。分离的化合物通过NMR分析阐明,质谱,和紫外光谱。使用真菌悬浮液研究抗真菌作用,孵化,和纯化化合物的寄生虫计数方法。使用肉汤稀释试验评估抗菌作用并根据MIC参数报告。
■两种名为鼠尾草酚的二萜化合物(化合物1),11-羟基-12-甲氧基-20-去甲-8,11,13-三烯(化合物2),和类黄酮:分离并鉴定6,7-二甲氧基-5,4'-二羟基黄酮(化合物3)。化合物1对白色念珠菌具有选择性抗真菌作用,C.光滑,和拟态梭菌,但对阴道毛滴虫的毒性较弱,IC50为675.8μg/mL,低于甲硝唑,IC50为13.2μg/mL。
■以鼠尾草为主要成分,曲霉菌,根霉,和毛滴虫物种。与标准药物相比,结果证实了其对念珠菌的作用。
UNASSIGNED: Salvia abrotanoides is considered a medicinal plant and has a broad distribution in Iran. In Iran\'s traditional medicine, it is also used to treat leishmaniasis, malaria, atherosclerosis, cardiovascular disease, and as a disinfectant. This research aimed to determine the anti-Candida component from S. abratonoides and anti-Trichomonas natural compounds from the stems of this plant.
UNASSIGNED: The plant shoots were collected, dried, and after removing the leaves, grounded. Dried plant material was extracted in a maceration tank, concentrated by a Rotavap, degreased, and fractionated by normal column chromatography. Based on anti-fungal screening against Candida species, Fr. 4, with more anti-fungal activity, was selected for phytochemical analysis, by different chromatographic methods on the silica gel column and Sephadex LH-20. Isolated compounds were elucidated by NMR analysis, mass spectrum, and ultraviolet spectroscopy. Anti-fungal effects were investigated using the fungal suspension, incubation, and parasite-counting methods on purified compounds. Antibacterial effects were assessed using the Broth dilution test and reported according to the MIC parameter.
UNASSIGNED: Two diterpenoid compounds named
carnosol (compound 1), 11-hydroxy-12-methoxy-20-norabiata-8, 11, 13-trien (compound 2), and a flavonoid: 6,7-dimethoxy-5, 4\'-dihydroxyflavone (compound 3) were isolated and identified. Compound 1 had selective anti-fungal effects against C. albicans, C. glabrata, and C. parapsilosis, but weak toxicity against Trichomonas vaginalis with IC50 of 675.8 μg/mL, less than metronidazole with an IC50 of 13.2 μg/mL.
UNASSIGNED: Carnosol as the main component was assayed against Candida, Aspergillus, Rhizopus, and Trichomanas species. The results confirmed its effect on Candida compared to standard drugs.