背景:肝细胞癌(HCC)是世界上最恶性的癌症之一,5年生存率低。目前,对于晚期原发性肝癌,临床治疗常采用全身方法,但是没有有效的靶向治疗。肝癌患者经过药物治疗后的平均生存时间仅为3-5个月。
目的:在这项研究中,我们旨在揭示鼠尾草酚对HCC的作用,为HCC的药物治疗提供新的可能性。
目的:观察鼠尾草酚对肝癌细胞肿瘤表型和信号通路的影响。
方法:我们处理了两种不同的人肝癌细胞,HepG2和Huh7,含鼠尾草酚。使用CCK-8测定法分析细胞的活力和增殖。通过Transwell实验检测细胞迁移和侵袭。细胞增殖的分子标志物,凋亡,迁移,入侵,并通过RT-PCR和WB检测信号通路。此外,我们用抑制剂进行了挽救实验,以验证受影响的信号通路.
结果:鼠尾草能显著抑制肝癌细胞的活力,努力,菌落形成,迁移,和入侵。此外,鼠尾草酚促进肝癌细胞凋亡。机械上,鼠尾草酚激活了AMPK-p53通路。
结论:总而言之,我们的研究表明,鼠尾草酚可以抑制增殖,迁移,入侵,并通过激活AMPK-p53促进肝癌细胞凋亡。
Hepatocellular carcinoma (HCC) is one of the most malignant cancers in the world, and its 5-
year survival rate is low. At present, for advanced primary liver cancer, the clinical treatment often adopts the systemic
method, but there is no effective targeted treatment. The average survival time of patients with liver cancer after drug
treatment is only 3-5 months. Therefore, it is of great clinical significance to find new and effective drugs for the
treatment of HCC.
Carnosol (CA) is a bioactive diterpene compound present in Lamiaceae spp., which has been
demonstrated to have antioxidant, anti-inflammatory, and anticancer properties.
In this study, we aimed to reveal the effect of
carnosol on HCC and provide new possibilities for the drug therapy
of HCC.
The objective of this study is to observe the effect of carnosol on the tumor phenotype and signaling pathway
of HCC cells.
We treated two different human HCC cells, HepG2 and Huh7, with
carnosol. The cells were analyzed using
the CCK-8 assay for viability and proliferation. The cell migration and invasion were detected by Transwell assay. The
molecular markers of cell proliferation, apoptosis, migration, invasion, and signaling pathways were detected by RTPCR
and WB. In addition, we performed rescue experiments with inhibitors to verify the affected signaling pathway.
The results showed that carnosol could significantly inhibit HCC cell viability, effort, colony formation, migration,
and invasion. Moreover,
Carnosol promoted the apoptosis of HCC cells. Mechanically,
carnosol activated the
AMPK-p53 pathway.
To conclude, our study demonstrated that carnosol could inhibit proliferation, migration, invasion, and
promote apoptosis via activating AMPK-p53 in HCC cells.