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Abstract：
The diphenolic diterpene carnosol was isolated from several species of the family Lamiaceae, including Lepechinia mutica, a medicinal plant endemic to Ecuador. The compound has exhibited high antioxidant, anti-inflammatory, antimicrobial, neuroprotective, and antifungal properties, as well as promising cytotoxicity against prostate, breast, skin, leukemia, and human colon cancer cell lines. In this paper, we developed and validated a simple, accurate, and reliable analytical HPLC-UV-ESI-IT-MS method, carried out on a C18 column, which is potentially suitable to quantify carnosol in plant extracts. The procedure complied with the established ICH validation parameters of analytical range (linearity in the range of 0.19-5.64 μg/g dried leaves; REAVERGE = 4.9%; R2 = 0.99907), analysis repeatability (RSD = 2.8-3.6%), intermediate precision (RSD = 1.9-3.6%), accuracy (estimated as % carnosol recovery in the range of 81 to 108%), and robustness. Finally, the LOD (0.04 µg/mg) and LOQ (0.19 μg/mg) values of carnosol/dried leaves were determined. Using this validated method, the content of carnosol in L. mutica was estimated to be 0.81 ± 0.04 mg/g of dried leaves (0.081%).
摘要：
从唇形科的几种物种中分离出二酚二萜鼠尾草酚，包括Lepechiniamutica，厄瓜多尔特有的一种药用植物。该化合物具有高抗氧化性能,抗炎,抗菌，神经保护,和抗真菌特性，以及对前列腺有希望的细胞毒性，乳房,皮肤,白血病,和人类结肠癌细胞系。在本文中,我们开发并验证了一个简单的，准确,和可靠的分析HPLC-UV-ESI-IT-MS方法，在C18色谱柱上进行，这可能适用于量化植物提取物中的鼠尾草酚。该程序符合已建立的ICH验证参数的分析范围（线性范围为0.19-5.64μg/g干叶；REAVERGE=4.9％；R2=0.99907），分析重复性(RSD=2.8-3.6%)，中间精度（RSD=1.9-3.6%），准确度(估计为81%至108%范围内的鼠尾草酚回收率)，和鲁棒性。最后,确定了鼠尾草/干叶的LOD（0.04µg/mg）和LOQ（0.19μg/mg）值。使用这种经过验证的方法，L.mutica中的鼠尾草酚含量估计为0.81±0.04mg/g干叶（0.081％）。
