目的:犬钩虫病是一种全球性的人畜共患寄生虫病,由钩虫科的多种线虫引起,包括环形目。,Necatorspp.,和Uncinariaspp。,在狗的小肠(主要是十二指肠)。该病在我国分布广泛。本研究的目的是通过小型化雪纳瑞犬饲料感染头孢霉素的病例,系统地诊断和治疗犬钩虫病。从而为后续犬钩虫病的防治提供实验依据。
方法:在目前的研究中,我们从一只患病的微型雪纳瑞犬中分离出钩虫卵,然后使用聚合酶链反应(PCR)从从钩虫中提取的基因组DNA中扩增ITS1-5.8S-ITS2基因序列。使用MEGA-X推断基于ITS1-5.8S-ITS2基因序列的系统发育分析。经过系统发育分析,病因学和对症疗法用于治疗犬钩虫病。
结果:测序结果表明,ITS1-5.8S-ITS2基因序列的长度约为960bp,并提取ITS1和ITS2与其他钩虫进行相似性分析,构建系统发育树。经过系统发育分析,结果表明,患病的小型雪纳瑞犬感染了塞利兰。使用病因和对症治疗,患有头孢霉素A.ceylanicum感染的病犬也被治疗5天。
结论:据我们所知,这是广州市首次报道犬钩虫病的诊断和治疗。此外,随着经济水平的提高,宠物狗养殖的规模也越来越大。本报告采用的诊断方法和治疗方案将有助于规范犬钩虫病的预防和控制。
OBJECTIVE: Canine hookworm disease is a global zoonotic parasitic disease caused by a variety of nematodes in families Ancylostomatidae, including Ancylostoma spp., Necator spp., and Uncinaria spp., in the small intestine (mainly the duodenum) of dogs. The disease is widely distributed in China. The purpose of this study is to systematically diagnose and treat canine hookworm disease through the case of miniaturization Schnauzer dog feed infected with A. ceylanicum, so as to provide experimental basis for subsequent prevention and control of canine hookworm disease.
METHODS: In the current study, we isolated hookworm eggs from a diseased miniature schnauzer, then the polymerase chain reaction (PCR) was used to amplify the ITS1-5.8S-ITS2 gene sequence from genomic DNA extracted from hookworms. Phylogenetic analysis based on ITS1-5.8S-ITS2 gene sequence sequences was inferred using MEGA-X. After phylogenetic analysis, etiologic and symptomatic therapies were used to treat the canine hookworm disease.
RESULTS: The sequencing results showed that the length of the ITS1-5.8S-ITS2 gene sequence was approximately 960 bp, and ITS1 and ITS2 were extracted to analyze similarity with other hookworms to build a phylogenetic tree. After phylogenetic analysis, the results showed that the diseased miniature schnauzer was infected by A. ceylanicum. Using etiologic and symptomatic therapies, the sick dog with an A. ceylanicum infection was also treated for 5 days.
CONCLUSIONS: To our knowledge, this is the first report of diagnosis and treatment for canine hookworm disease in Guangzhou city. In addition, with the improvement of economic level, the scale of pet dog breeding is also increasing. The diagnostic methods and treatment schemes adopted in this report will help to standardize the prevention and control of canine hookworm disease.