PDF(Pubmed)
Abstract：
Ancylostoma ceylanicum is a zoonotic soil-derived nematode that parasitizes the intestines of humans and animals (dogs and cats), leading to malnutrition and iron-deficiency anemia. Helminth parasites secrete calreticulin (CRT), which regulates or blocks the host\'s immune response. However, no data on A. ceylanicum calreticulin (Ace-CRT) are available. We investigated the biological function of recombinant Ace-CRT (rAce-CRT). rAce-CRT showed reliable antigenicity and stimulated the proliferation of mouse splenocytes and canine peripheral blood mononuclear cells. Quantitative reverse-transcription PCR assays revealed that rAce-CRT primarily promoted the expression of T helper 2 cytokines, particularly IL-13, in canine peripheral blood lymphocytes. rAce-CRT inhibited complement-mediated sheep erythrocyte hemolysis in vitro. Our findings indicate that Ace-CRT plays an immunomodulatory role and may be a promising candidate molecule for a hookworm vaccine.
摘要：
Ancylostomaceylanicum是一种人畜共患的土壤线虫，寄生在人类和动物（狗和猫）的肠道中，导致营养不良和缺铁性贫血。蠕虫寄生虫分泌钙网蛋白（CRT），调节或阻断宿主的免疫反应。然而,没有关于ceylanicum钙网蛋白（Ace-CRT）的数据。我们研究了重组Ace-CRT（rAce-CRT）的生物学功能。rAce-CRT显示出可靠的抗原性，并刺激小鼠脾细胞和犬外周血单个核细胞的增殖。定量逆转录PCR检测显示，rAce-CRT主要促进T辅助细胞因子2的表达，特别是IL-13，在犬外周血淋巴细胞。rAce-CRT在体外抑制补体介导的绵羊红细胞溶血。我们的发现表明Ace-CRT起着免疫调节作用，可能是钩虫疫苗的有希望的候选分子。
