Overuse of sulfonamides in aquaculture and agriculture leads to residual drugs that cause serious pollution of the environment. However, the residues of sulfonamides in the environment are not unique, and the existing microbial degradation technology has a relatively low degradation rate of sulfonamides. Therefore, in this study, a Pseudomonas stutzeri strain (DLY-21) with the ability to degrade four common SAs was screened and isolated from aerobic compost. Under optimal conditions, the DLY-21 strain degraded four sulfonamides simultaneously within 48 h, and the degradation rates were all over 90%, with the average degradation rates of SAs being sulfoxide (SDM) ≈ sulfachloropyridazine (SCP) > sulfa quinoxaline (SQ) > sulfadiazine (SQ). In addition, the main compounds of the strain DLY-21-degrading SAs were identified by LC-MS analysis. On this basis, four detailed reaction pathways for SA degradation were deduced. This is the first report of the use of a P. stutzeri strain to degrade four sulfonamide antibiotics (SQ, SDM, SCP, and SM1), which can improve the removal efficiency of sulfonamide antibiotic pollutants and thus ameliorate environmental pollution. The results showed that DLY-21 had a good degradation effect on four SAs (SQ, SDM, SCP, and SM1).

OBJECTIVE: With the development of animal husbandry in China, the production of a large amount of livestock and poultry manure has become one of the main agricultural pollution sources. High-temperature aerobic composting stands out as one of the most crucial methods for the safe and resourceful utilization of livestock and poultry manure, serving as an essential link between crop cultivation, animal breeding, and sustainable agricultural development. Numerous studies have demonstrated that the addition of exogenous multifunctional bacterial agents to compost reduces not only harmful emissions but also sequesters or increases essential nutrients. However, these efficacies depend on the specific functions of the bacteriophage itself, the harmonization and complementarity within the colony, and its ability to adapt to the environment. In recent years, relatively few studies have been conducted on actinomycetes. This experiment provides excellent actinomycete resources for the production of high-efficiency and high-quality compost compound microbial agents of manure and straw.

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) has gained attention as a possible substitute for conventional polymers that could be integrated into the organic recycling system. Biocomposites with 15% of pure cellulose (TC) and woodflour (WF) were prepared to analyze the role of lignin on their compostability (58 °C) by tracking the mass loss, CO2 evolution, and the microbial population. Realistic dimensions for typical plastic products (400 µm films), as well as their service performance (thermal stability, rheology), were taken into account in this hybrid study. WF showed lower adhesion with the polymer than TC and favored PHBV thermal degradation during processing, also affecting its rheological behavior. Although all materials disintegrated in 45 days and mineralized in less than 60 days, lignin from woodflour was found to slow down the bioassimilation of PHBV/WF by limiting the access of enzymes and water to easier degradable cellulose and polymer matrix. According to the highest and the lowest weight loss rates, TC incorporation allowed for higher mesophilic bacterial and fungal counts, while WF seemed to hinder fungal growth. At the initial steps, fungi and yeasts seem to be key factors in facilitating the later metabolization of the materials by bacteria.

Hyperspectral technology, with its high spectrum resolution and nanometer continuous spectral information acquisition ability, provide a possibility for rapidly and nondestructive evaluating compost maturity. In this study, the near-infrared spectroscopy (NIRS) analysis techniques was used to analyze quantitatively organic matter (OM) content, total nitrogen (TN) content and carbon-nitrogen (C/N) ratio in compost based on two different composting procedures. In the basis of spectra preprocessing and strategies of variable selection, the nonlinear modeling LBC-siPLS-PLSR for OM, MSC-SPA-PLSR for TN and R-SPA-PLSR for C/N ratio was respectively constructed using partial least squares regression (PLSR). LBC-siPLS-PLSR, MSC-SPA-PLSR and R-SPA-PLSR provided a better prediction capability with root mean square error of prediction, the coefficient of determination for prediction and residual predictive deviation values of 4.061, 0.746 and 2.02 for OM, values of 0.205, 0.65 and 1.71 for TN and values of 1.11, 0.706 and 2.07 for C/N ratio, respectively. These results showed that the NIRS technique could be fitted to each element, using specific spectrum pretreatment, in order to achieve an acceptable accuracy in the prediction.

Composting is an effective method of recycling organic solid waste, and it is the key process linking planting with recycling. To explore the reuse of agricultural organic solid waste as a resource in the Yellow River Delta, the effects of microbial inoculant and different additives (calcium superphosphate, biochar, tomato straw, rice husk, and sugar residue) on pile composting of cow dung were studied to obtain the best composting conditions. The results showed that microbial inoculant and additives all played positive roles in the process of aerobic composting, and the experimental groups outperformed the control groups without any additives. For discussion, the microbial inoculant promoted rapid pile body heating more than the recovery materials alone, and the effects on aerobic composting were related to the organic matter of substrates and biochar. After being composted, all the materials were satisfactorily decomposed. Degradation of additives into humic acid might serve as electron shuttles to promote thorough organic matter decomposition. These results provide a scientific basis data for industrial composting of organic solid waste processed by on-site stacking, and provide a reference for researcher and practitioners for studying the applications of microbial inoculant on aerobic composting.

Cyanobacterial bloom is by far one of the most common water quality hazards. As cyanobacteria are rich in nitrogen, phosphorus, and other organic matter, the potential for beneficial use of cyanobacteria is promising. Aerobic composting is currently a hot topic of research in cyanobacteria treatment, which can effectively achieve reduction, recycling, and removal of the harmful impact of cyanobacteria. In this review, the characteristics of cyanobacteria in aerobic composting processes, the effects of physical, chemical, and biological factors on the composting process, and the degradation of microcystic toxins were systematically discussed and summarized. This review epitomizes the large quantities of research data collected by many scholars around the world to address the characteristics of \"one low and five highs\" in the aerobic cyanobacterial composting process. The composting techniques developed are effective and easy to adopt in the real world, such as adjusting the substrate C/N ratio and moisture content and use of chemical and biological additives to achieve reduction, recycling, and detoxication of the cyanobacterial wastes. The aim of this comprehensive review is to provide theoretical guidance and reference for further development and application of aerobic cyanobacteria composting technology.

Humic substances (HSs) occupy 80% of organic matter in soil and have been widely applied for soil remediation agents, potential battery materials, and adsorbents. Since the HS extraction rate is very low by microbial degradation in nature, artificial humification processes such as aerobic composting (AC) and hydrothermal treatment (HT) have attracted a great deal of attention as the most important strategies in HS production. This article aims to provide a state-of-the-art review on the development of conversion of biomass waste into HSs based on AC and HT for the first time in terms of mechanisms, characteristics of HSs\' molecular structure, and influencing factors. In addition, some differences based on the aforementioned information between AC and HT are reviewed and discussed in the conversion of biomass waste into HSs in a pioneering way. For biomass waste conversion, a feasible strategy on effective humification processes by combining AC with HT is proposed.

The disposal of organic waste by the biocomposting of black soldier fly larvae (BSFL) has drawn broad attention. However, the discrepancies in heavy metal immobilization between BSFL biocomposting with different inoculation densities and aerobic composting need to be further researched. In this study, BSFL with inoculation densities of 0.08%, 0.24% and 0.40% was added to swine manure to investigate its influence on heavy metal bioaccumulation and bioavailability. The physicochemical properties, BSFL growth performance and amino acid contents were measured. The results showed that the germination index, total prepupal yield and bioavailable fraction removal rate (%) of Cr and Pb at an inoculation density of 0.40% of BSFL were the highest among all of the BSFL biocomposting groups. Although the bioaccumulation factor and heavy metal (Cd, Cr, Cu and Zn) concentrations of the BSFL body from swine manure with inoculation densities of 0.24% and 0.40% of BSFL were similar, the BSFL inoculation density of 0.40% had the best absorption effect on these heavy metals in terms of total prepupal yield. Therefore, this study provides a basis for exploring the optimal inoculation density of BSFL biocomposting to reduce the harmful effects of heavy metals in swine manure.

The pertinent challenges associated with effective treatment of fecal sludge in medium scales necessitate alternative means for land application. The methods of compost preparation from sewage sludge and their modes of application to the agricultural fields have profound impacts on the soil ecology and environment. Besides the chemical conditioning effects on soil organic matter, they also impart physical attributes to the soil texture and structure. Though it is expected that compost addition improves water holding capacity and nutrient sequestration, there is lack of clarity in correlating the field outcomes with conditions of excess nutrient storage/leaching despite the agronomic benefits. In this study, we present a systematic cause-evidence-impact relationship on the feedstock composition, processing, and applications of co-composted sewage sludge. Various analytical tools were compared to elucidate the unique characteristics of co-composted sewage sludge to get a realistic understanding of the complex soil-compost interactions. Results from the spectroscopic characterization reveal the implications of selection of bulking agents and sludge pre-treatment in determining the final quality of the compost. Based on the results, we postulate a unique attribution of parent material influence to the formation of well-defined porous structures which influences the nutrient leaching/sequestrating behavior of the soil. Thus, the compounded impacts of composted organic matter on the soil and crop can be proactively determined in terms of elemental composition, functional groups, and stability indices. The present approach provides good scope for customizing the preparations and applications of aerobic microbial composts in order to derive the preferred field outputs.

The new sugar source manno-oligosaccharide can regulate the structure of the microbial community. This study investigated the effects of adding manno-oligosaccharide at four different levels (0, 0.1%, 0.5%, and 1% w/w compost) to composting cow manure and straw on lignocellulose degradation and the bacterial community. Adding 0.5% manno-oligosaccharide had the greatest effects on accelerating the composting process, reducing its toxicity, and improving the stability of the product. After composting for 25 days, adding 0.5% manno-oligosaccharide decreased the hemicellulose, cellulose, and lignin contents to 2.25%, 11.25%, and 7.07%, respectively, compared with those under CK. Manno-oligosaccharide promoted the degradation of lignocellulose by increasing the abundances of Thermobifida, Streptomyces, and Luteimonas. In addition, manno-oligosaccharide inhibited pathogenic bacteria and increased the abundances of functional genes related to metabolism. Finally, adding 0.5% manno-oligosaccharide mainly affected the degradation of lignocellulose by enhancing the C/N ratio and the abundances of Streptomyces and the secretion system during composting according to redundancy analysis.