PDF(Pubmed)
Abstract：
Overuse of sulfonamides in aquaculture and agriculture leads to residual drugs that cause serious pollution of the environment. However, the residues of sulfonamides in the environment are not unique, and the existing microbial degradation technology has a relatively low degradation rate of sulfonamides. Therefore, in this study, a Pseudomonas stutzeri strain (DLY-21) with the ability to degrade four common SAs was screened and isolated from aerobic compost. Under optimal conditions, the DLY-21 strain degraded four sulfonamides simultaneously within 48 h, and the degradation rates were all over 90%, with the average degradation rates of SAs being sulfoxide (SDM) ≈ sulfachloropyridazine (SCP) > sulfa quinoxaline (SQ) > sulfadiazine (SQ). In addition, the main compounds of the strain DLY-21-degrading SAs were identified by LC-MS analysis. On this basis, four detailed reaction pathways for SA degradation were deduced. This is the first report of the use of a P. stutzeri strain to degrade four sulfonamide antibiotics (SQ, SDM, SCP, and SM1), which can improve the removal efficiency of sulfonamide antibiotic pollutants and thus ameliorate environmental pollution. The results showed that DLY-21 had a good degradation effect on four SAs (SQ, SDM, SCP, and SM1).
摘要：
在水产养殖和农业中过度使用磺胺类药物会导致残留药物，从而对环境造成严重污染。然而,环境中的磺胺类药物残留并不独特，现有的微生物降解技术对磺胺类药物的降解率相对较低。因此,在这项研究中，筛选并从好氧堆肥中分离出具有降解四种常见SA能力的Stutzeri假单胞菌菌株（DLY-21）。在最优条件下,DLY-21菌株在48h内同时降解4种磺胺类药物，降解率均超过90%，SAs的平均降解率为亚砜(SDM)≈磺胺喹喔啉(SQ)>磺胺嘧啶(SQ)。此外,通过LC-MS分析鉴定了菌株DLY-21降解SAs的主要化合物。在此基础上,推导出了四种降解SA的详细反应途径。这是首次报道使用Stutzeri菌株降解四种磺胺类抗生素（SQ，SDM,SCP,和SM1），可以提高磺胺类抗生素污染物的去除效率，从而改善环境污染。结果表明,DLY-21对四种SAs(SQ,SDM,SCP,和SM1）。
