■氧化锌纳米颗粒(ZnO-NP)在生物医学研究中引起了相当大的兴趣,这主要是由于它们在对抗致病性疾病和微生物感染方面的前瞻性治疗意义。这项研究的主要目的是使用鸡蛋清(白蛋白)作为生物模板检查氧化锌纳米晶须(ZnO-NWs)的生物合成。此外,本研究旨在探索ZnONWs在感染性疾病中的潜在生物医学应用。
■使用电子显微镜观察通过生物过程合成的NWs,这允许详细检查它们的特征。这些研究的结果表明,NW的尺寸分布范围约为10至100nm。傅里叶变换红外光谱(FTIR)和扫描电子显微镜-能量色散X射线光谱(SEM-EDX)映射分析成功地证实了尺寸,尺寸,以及生物成分在其形成过程中的存在。在这项研究中,采用XTT测定和共聚焦成像来提供ZnO-NW在根除细菌生物膜中的功效的证据。目标菌株为金黄色葡萄球菌和大肠杆菌。此外,我们试图解决有关ZnO-NWs生物相容性的相关问题。这是通过对正常HEK-293T和红细胞中不存在细胞毒性的综合评价来实现的。
■这项研究的结果明确证实了ZnO-NWs的生物相容性。在持续一周的治疗干预后,生物合成的ZnO-NW表现出显著的减轻小鼠模型中金黄色葡萄球菌诱导的皮炎诱导后果的能力。
■这项研究提出了对源自鸡蛋清的氧化锌纳米晶须(ZnO-NWs)的生物合成的全面检查。这些发现强调了生物合成的ZnO-NWs作为开发针对传染病的治疗剂的可行选择的巨大潜力。ZnO-NWs对敏感和抗生素耐药菌株的抗菌功效,以及它们根除生物膜的能力,表明了它们在抗击传染病方面的有希望的作用。此外,证实的ZnO-NWs的生物相容性为其在生物医学应用中的安全使用开辟了道路。总的来说,这项研究强调了ZnO-NWs的治疗前景及其在未来生物医学进步中的潜在意义。
Zinc oxide nanoparticles (ZnO-NPs) have garnered considerable interest in biomedical research primarily owing to their prospective therapeutic implications in combatting pathogenic diseases and microbial infections. The primary objective of this study was to examine the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) using chicken egg white (albumin) as a bio-template. Furthermore, this study aimed to explore the potential biomedical applications of ZnO NWs in the context of infectious diseases.
The NWs synthesized through biological processes were observed using electron microscopy, which allowed for detailed examination of their characteristics. The results of these investigations indicated that the NWs exhibited a size distribution ranging from approximately 10 to 100 nm. Fourier-transform infrared spectroscopy (FTIR) and scanning electron microscopy-energy dispersive X-ray spectroscopy (SEM-EDX) mapping analyses successfully corroborated the size, dimensions, and presence of biological constituents during their formation. In this study, XTT assay and confocal imaging were employed to provide evidence of the efficacy of ZnO-NWs in the eradication of bacterial biofilms. The target bacterial strains were Staphylococcus aureus and Escherichia coli. Furthermore, we sought to address pertinent concerns regarding the biocompatibility of the ZnO-NWs. This was achieved through comprehensive evaluation of the absence of cytotoxicity in normal HEK-293T and erythrocytes.
The findings of this investigation unequivocally confirmed the biocompatibility of the ZnO-NWs. The biosynthesized ZnO-NWs demonstrated a noteworthy capacity to mitigate the dermatitis-induced consequences induced by Staphylococcus aureus in murine models after a therapeutic intervention lasting for one week.
This study presents a comprehensive examination of the biosynthesis of zinc oxide nanowhiskers (ZnO-NWs) derived from chicken egg whites. These findings highlight the considerable potential of biosynthesized ZnO-NWs as a viable option for the development of therapeutic agents targeting infectious diseases. The antibacterial efficacy of ZnO-NWs against both susceptible and antibiotic-resistant bacterial strains, as well as their ability to eradicate biofilms, suggests their promising role in combating infectious diseases. Furthermore, the confirmed biocompatibility of ZnO-NWs opens avenues for their safe use in biomedical applications. Overall, this research underscores the therapeutic promise of ZnO-NWs and their potential significance in future biomedical advancements.