PDF(Pubmed)
Abstract：
UNASSIGNED: Infectious skin diseases are a type of inflammatory skin lesions caused by pathogenic microorganisms. Because of the uncertainty of methodology, the skin infection model usually have low replication rate and lack of good evaluation system. We aimed to establish multi-index and comprehensive evaluation method for Staphylococcus aureus (S.aureus) skin-infection models through Analytic hierarchy process (AHP) and Delphi method, and screen high quality animal models through it.
UNASSIGNED: Firstly, the evaluation indicators of skin infection were collected basing on literature research. The weight of the evaluation indicators were decided according to AHP and Delphi method. Then different ulcer models (mouse or rat) infected by S. aureus were selected as the research objects.
UNASSIGNED: The evaluation indicators were classified into four groups of criteria (including ten sub-indicators) and given different weights, physical sign changes (0.0518), skin lesion appearance (0.2934), morphological observation (0.3184), etiological examination (0.3364). Through the evaluation system, we screened and found that the mouse ulcer model which caused by a round wound and 1.0 × 1010 CFU/mL (0.1 mL) bacterial concentration got the highest comprehensive score, and also found that the model which caused by a 1.5 cm-round wound and 1.0 × 1010 CFU/mL (0.2 mL) maybe the best rat ulcer model.
UNASSIGNED: This study has established an evaluation system based on AHP and Delphi method, also provided the best skin ulcer models selected by this system, the models are suitable for disease research and drug development research of skin ulcer.
UNASSIGNED: Firstly, the evaluation indicators of skin infection were collected basing on literature research. The weight of the evaluation indicators were decided according to AHP and Delphi method. Then different ulcer models (mouse or rat) infected by S. aureus were selected as the research objects.
UNASSIGNED: The evaluation indicators were classified into four groups of criteria (including ten sub-indicators) and given different weights, physical sign changes (0.0518), skin lesion appearance (0.2934), morphological observation (0.3184), etiological examination (0.3364). Through the evaluation system, we screened and found that the mouse ulcer model which caused by a round wound and 1.0 × 1010 CFU/mL (0.1 mL) bacterial concentration got the highest comprehensive score, and also found that the model which caused by a 1.5 cm-round wound and 1.0 × 1010 CFU/mL (0.2 mL) maybe the best rat ulcer model.
UNASSIGNED: This study has established an evaluation system based on AHP and Delphi method, also provided the best skin ulcer models selected by this system, the models are suitable for disease research and drug development research of skin ulcer.
摘要：
■传染性皮肤病是由病原微生物引起的一类炎症性皮肤病变。由于方法论的不确定性,皮肤感染模型复制率低，缺乏良好的评价体系。我们旨在建立金黄色葡萄球菌的多指标综合评价方法(S.金黄色葡萄球菌)通过层次分析法(AHP)和德尔菲法建立皮肤感染模型，并通过它筛选高质量的动物模型。
■首先，在文献研究的基础上收集皮肤感染的评价指标。根据层次分析法和德尔菲法确定评价指标的权重。然后选择金黄色葡萄球菌感染的不同溃疡模型(小鼠或大鼠)作为研究对象。
■将评价指标分为四组标准（包括十个子指标），并赋予不同的权重，体征变化（0.0518），皮肤病变外观（0.2934），形态学观察（0.3184），病因检查（0.3364）。通过评价体系,我们筛选发现，圆形伤口和1.0×1010CFU/mL（0.1mL）细菌浓度引起的小鼠溃疡模型的综合评分最高，并发现由1.5cm圆形伤口和1.0×1010CFU/mL（0.2mL）引起的模型可能是最佳的大鼠溃疡模型。
■本研究建立了基于层次分析法和德尔菲法的评价体系，还提供了该系统选择的最佳皮肤溃疡模型，该模型适用于皮肤溃疡的疾病研究和药物开发研究。
■首先，在文献研究的基础上收集皮肤感染的评价指标。根据层次分析法和德尔菲法确定评价指标的权重。然后选择金黄色葡萄球菌感染的不同溃疡模型(小鼠或大鼠)作为研究对象。
■将评价指标分为四组标准（包括十个子指标），并赋予不同的权重，体征变化（0.0518），皮肤病变外观（0.2934），形态学观察（0.3184），病因检查（0.3364）。通过评价体系,我们筛选发现，圆形伤口和1.0×1010CFU/mL（0.1mL）细菌浓度引起的小鼠溃疡模型的综合评分最高，并发现由1.5cm圆形伤口和1.0×1010CFU/mL（0.2mL）引起的模型可能是最佳的大鼠溃疡模型。
■本研究建立了基于层次分析法和德尔菲法的评价体系，还提供了该系统选择的最佳皮肤溃疡模型，该模型适用于皮肤溃疡的疾病研究和药物开发研究。
