■从事肛交显着增加与男性发生性关系的男性(MSM)中感染艾滋病毒的风险;口交活动倾向于独特地将肠道微生物引入唾液微生物群,which,结合个人的艾滋病毒阳性状况,可能会极大地扰乱口腔微生态。然而,直到日期,只有少数已发表的研究针对这方面。
■基于细菌分类群的16SrRNA测序数据,MicroPITA挑选代表性样本进行宏基因组分析,有效揭示HIV疾病的发展和进展如何影响MSM的口腔微生物群。因此,我们收集了11名HIV阴性和44名HIV阳性MSM受试者的样本(第0阶段由HIVRNA阳性定义,但抗体状态为阴性或不确定;第1、2和3期定义为CD4+T淋巴细胞计数≥500、200-499和≤200或机会性感染),并使用MicroPITA选择了25份代表性唾液样本(5例/期)。进行宏基因组测序分析以探索HIV阳性状态是否改变MSM中的唾液细菌KEGG功能和代谢途径。
■五组均维持口腔微生物群的核心功能,包括新陈代谢,遗传和环境信息处理。所有HIV阳性者均表现出异常增殖的KEGG功能,最突出的是在0阶段,其他与新陈代谢有关。聚类关系分析初步确定了群体之间的功能关系,0期对照组和1-2期对照组的细菌功能更相似,而3期组表现出较大的功能变化。尽管我们确定了所有五组的大多数代谢途径都是常见的,几个独特的途径形成了某些群体的集群;0期组有几个,而第二阶段和第三阶段的小组很少,这样的集群。K03046的丰度与CD4计数呈正相关。
■随着HIV的发展,MSM的唾液细菌功能和代谢途径逐渐变化,这可能与HIV促进异常能量代谢和加剧病原体毒力有关。Further,艾滋病急性期和免疫细胞破坏异常增加,预测MSM个体发生全身性和口腔疾病的风险增加。
UNASSIGNED: Engaging in anal sexual intercourse markedly increases the risk of developing HIV among men who have sex with men (MSM); oral sexual activities tend to uniquely introduce gut-derived microbes to salivary microbiota, which, combined with an individual\'s positive HIV status, may greatly perturb oral microecology. However, till date, only a few published studies have addressed this aspect.
UNASSIGNED: Based on 16S rRNA sequencing data of bacterial taxa, MicroPITA picks representative samples for metagenomic analysis, effectively revealing how the development and progression of the HIV disease influences oral microbiota in MSM. Therefore, we collected samples from 11 HIV-negative and 44 HIV-positive MSM subjects (stage 0 was defined by HIV RNA positivity, but negative or indeterminate antibody status; stages 1, 2, and 3 were defined by CD4+ T lymphocyte counts ≥ 500, 200-499, and ≤ 200 or opportunistic infection) and selected 25 representative saliva samples (5 cases/stage) using MicroPITA. Metagenomic sequencing analysis were performed to explore whether positive HIV status changes salivary bacterial KEGG function and metabolic pathway in MSM.
UNASSIGNED: The core functions of oral microbiota were maintained across each of the five groups, including metabolism, genetic and environmental information processing. All HIV-positive groups displayed KEGG functions of abnormal proliferation, most prominently at stage 0, and others related to metabolism. Clustering relationship analysis tentatively identified functional relationships between groups, with bacterial function being more similar between stage 0-control groups and stage 1-2 groups, whereas the stage 3 group exhibited large functional changes. Although we identified most metabolic pathways as being common to all five groups, several unique pathways formed clusters for certain groups; the stage 0 group had several, while the stage 2 and 3 groups had few, such clusters. The abundance of K03046 was positively correlated with CD4 counts.
UNASSIGNED: As HIV progresses, salivary bacterial function and metabolic pathways in MSM progressively changes, which may be related to HIV promoting abnormal energy metabolism and exacerbate pathogen virulence. Further, infection and drug resistance of acute stage and immune cell destruction of AIDS stage were abnormally increased, predicting an increased risk for MSM individuals to develop systemic and oral diseases.