背景:酵母培养物(YC)是在特定培养基上发酵的产品,这是一种厌氧固态发酵的后生物。虽然YC对动物的生长和健康有积极的影响,它含有多种有益的代谢产物作为暗物质,尚未量化。在本研究中,液相色谱-串联质谱法用于鉴定未知代谢物。在他们的身份之后,使用HPLC-二极管阵列检测方法对重要的化学物质进行定量。
结果:非靶向代谢组学研究表明,YC中总共鉴定出670种代谢物,其中23种代谢物显著增加,包括有机酸,氨基酸,核苷和嘌呤,异黄酮,和其他物质。化学定量分析表明,琥珀酸的含量,氨基丁酸,谷氨酰胺,嘌呤和大豆苷元增加了84.42%,51.07%,100%,68.85%和4.60%,分别。
结论:因此,利用非靶向代谢组学结合化学定量分析来揭示YC的营养和功能物质,有助于阐明菌后机制,为调控有益代谢产物的定向积累提供理论支持。©2024化学工业学会。
BACKGROUND: Yeast culture (YC) is a product fermented on a specific medium, which is a type of postbiotic of anaerobic solid-state fermentation. Although YC has positive effects on the animal growth and health, it contains a variety of beneficial metabolites as dark matter, which have not been quantified. In the present study, liquid chromatography-tandem mass spectrometry is employed to identify the unknown metabolites. Following their identification, the important chemicals are quantified using HPLC-diode array detection methods.
RESULTS: Non-targeted metabolomics studies showed that 670 metabolites in total were identified in YC, of which 23 metabolites significantly increased, including organic acids, amino acids, nucleosides and purines, isoflavones, and other substances. The chemical quantitative analysis showed that the contents of succinic acid, aminobutyric acid, glutamine, purine and daidzein increased by 84.42%, 51.07%, 100%, 68.85% and 4.60%, respectively.
CONCLUSIONS: Therefore, the use of non-targeted metabolomics combined with chemical quantitative analysis to reveal the nutritional and functional substances of YC could help to elucidate the postbiotic mechanism and provide theoretical support for the regulation of the directional accumulation of beneficial metabolites. © 2024 Society of Chemical Industry.