organic acids

有机酸
  • 文章类型: Journal Article
    烧烤食品是丙烯酰胺的重要来源,具有神经毒性,基因毒性,和致癌特性。本研究旨在评估牛肉中丙烯酰胺的含量,鸡肉,和鱼产品,尤其是那些需要高烹饪温度的人,使用高效液相色谱(HPLC)。通过有机酸减少丙烯酰胺,即(柠檬酸,苹果酸,酒石酸,和乳酸)和柠檬的水果提取物,苹果,葡萄也被调查过。结果表明,在鸡肉产品(烤鸡)中发现最高的平均丙烯酰胺浓度,记录为8.32μg/100g,其次是牛肉产品(牛肉烤),浓度为7.91μg/100g,和记录为6.77μg/100g的鱼产品(炸鱼汉堡)。此外,在化学模型系统中,有机酸混合物对降低丙烯酰胺含量的作用最大。此外,水果提取物混合物比有机酸混合物更有效地降低烤鸡中丙烯酰胺的百分比。最后,水果提取物的添加改善了烤鸡的感官特性。总之,这项研究提供了新颖且有前途的天然策略,以减少肉类产品中的丙烯酰胺,从而进一步应用于肉类行业,为消费者提供安全的食品。
    Grilled foods are an important source of acrylamide, which has neurotoxic, genotoxic, and carcinogenic properties. The current study aims to evaluate the level of acrylamide in beef, chicken, and fish products, especially those requiring high cooking temperatures, using High Performance Liquid Chromatography (HPLC). Reduction of acrylamide by organic acids i.e., (citric acid, malic acid, tartaric acid, and lactic acid) and fruit extracts of lemon, apple, and grape has also been investigated. The results revealed that the highest mean acrylamide concentration was found in chicken products (grilled chicken) which recorded 8.32 μg/100 g, followed by beef products (beef grilled) with a concentration of 7.91 μg/100 g, and fish products (pan-fried fish burgers) which recorded 6.77 μg/100 g). Furthermore, the mixture of organic acid has the highest effect on reducing the level of acrylamide in a chemical model system. Moreover, the fruit extract mixture was more effective in reducing the percentage of acrylamide in the grilled chicken than organic acids mixture. Finally, the addition of fruit extract improved the sensory properties of grilled chickens. In sum, this study offers novel and promising natural strategies to decrease acrylamide in meat products toward further future application in meat industry to deliver safe food to consumers.
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  • 文章类型: Journal Article
    千百年来,酒精饮料发展出独特的风味,酸味在他们的感官知觉和质量中起着至关重要的作用。有机酸,作为关键的风味化合物,显著影响风味。本文综述了酒精饮料中酸味和关键有机酸的感官属性。关于酸味,研究方法包括静态和动态感官方法,总结了酸味与香气的相互作用,味道,和口感。此外,这篇综述的重点是识别关键的有机酸,包括样品提取,色谱,嗅觉/味道,和质谱。酒精饮料中的关键有机酸,比如葡萄酒,白酒,啤酒,还有黄九,并讨论了它们的主要监管方法。最后,通过耦合机器学习探索酸味和有机酸的未来途径,数据库,建议使用感觉相互作用和脑电图。本系统综述旨在加深对酒精饮料的理解,并为进一步深入研究提供参考。
    Alcoholic beverages have developed unique flavors over millennia, with sourness playing a vital role in their sensory perception and quality. Organic acids, as crucial flavor compounds, significantly impact flavor. This paper reviews the sensory attribute of sour flavor and key organic acids in alcoholic beverages. Regarding sour flavor, research methods include both static and dynamic sensory approaches and summarize the interaction of sour flavor with aroma, taste, and mouthfeel. In addition, this review focuses on identifying key organic acids, including sample extraction, chromatography, olfactometry/taste, and mass spectrometry. The key organic acids in alcoholic beverages, such as wine, Baijiu, beer, and Huangjiu, and their primary regulatory methods are discussed. Finally, future avenues for the exploration of sour flavor and organic acids by coupling machine learning, database, sensory interactions and electroencephalography are suggested. This systematic review aims to enhance understanding and serve as a reference for further in-depth studies on alcoholic beverages.
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  • 文章类型: Journal Article
    有机酸(OAs)由于其调节胃肠环境的能力和其抗微生物能力而用于动物饲料中以调节胃肠疾病和腹泻。然而,关于最适当的OA及其在兔养殖中的有效性,没有足够的证据。因此,这项研究的目的是筛选和评估幼兔对六种OAs的反应,通过饮用水管理,在三个不同的浓度(pH水平)。有机酸(乙酸,ACET;甲酸,对于;丙酸,道具;乳酸,LAC;柠檬酸,CIT;和丁酸,BUT)在三种浓度(pH3、4和5)下进行了测试。还包括阴性对照(CON;非酸化水)。我们使用240只断奶兔(28日龄),分为2批。在每个批次中,将动物随机分配到19种实验治疗中的1种,并安置在每个笼子6只动物的笼子中,治疗,和批处理。在240只兔子中,包括具有6只动物的另外的笼子以确定动物的初始生理状态。在整个实验中,所有动物都用商业颗粒饲料喂养。研究的持续时间为7天,直到35天的年龄。在31和35天大的时候,在每个批次中,每天宰杀三只动物,并进行治疗。眼底消化内容物的pH值,胃窦,十二指肠,空肠,回肠,和盲肠,以及胃蛋白酶活性,是测量的。每天记录每笼的水和饲料消耗以及个体体重(BW)。OAs的种类和用量影响取水量。与CON相比,ACET3,PROP3和BUT3减少了水的摄入量,对饲料摄入量和体重增加产生负面影响。对于和CIT酸导致35天的最高体重和体重增加,与PROP相比,LAC,和BUT(p<0.05);显示ACET中间值。虽然OAs对胃和小肠pH的影响有限,与pH3相比,pH4和5的酸化水降低了回肠和盲肠的pH(p<0.05)。与pH3和pH5相比,pH4的酸化水显示出最高的(p<0.05)胃蛋白酶活性。考虑到我们筛选测试的样本量有限和短期评估期,在断奶后兔子中使用潜力最大的OAs是FOR,ACET,和pH为4的CIT。所选择的组合在幼兔中没有表现出任何早期副作用。这些结果应该在更广泛的动物群体中得到进一步证实。还建议在更长的时间内延长OAs的应用,以评估其在兔子整个生长期的作用。
    Organic acids (OAs) are employed in animal feed to regulate gastrointestinal disorders and diarrhoea thanks to their ability to modulate the gastrointestinal environment and their antimicrobial capacity. However, there is not enough evidence regarding the most adequate OA and its effectiveness in rabbit farming. Therefore, the aim of this study was to screen and evaluate the response of young rabbits to six OAs, administered via drinking water, at three different concentrations (pH levels). Organic acids (acetic, ACET; formic, FOR; propionic, PROP; lactic, LAC; citric, CIT; and butyric, BUT) were tested at three concentrations (pH 3, 4, and 5). A negative control (CON; non-acidified water) was also included. We used 240 weaned rabbits (28 days old) divided into 2 batches. In each batch, animals were randomly allocated to 1 of the 19 experimental treatments and were housed in group cages of 6 animals per cage, treatment, and batch. Among the 240 rabbits, an additional cage with 6 animals was included to determine the initial physiological state of the animals. All animals were fed with commercial pelleted feed throughout the whole experiment. The duration of the study was 7 days, until 35 days of age. At 31 and 35 days of age, in each batch, three animals per day and treatment were slaughtered. The pH of the digestive contents in the fundus, antrum, duodenum, jejunum, ileum, and cecum, as well as the gastric pepsin enzyme activity, was measured. Water and feed consumption per cage and individual body weight (BW) were recorded daily. The type and dosage of OAs affected water intake. ACET 3, PROP 3, and BUT 3 reduced water intake compared to CON, negatively impacting feed intake and weight gain. FOR and CIT acids led to the highest BW and weight gain at 35 days, compared to PROP, LAC, and BUT (p < 0.05); showing ACET intermediate values. While OAs had limited effects on gastric and small intestine pH, acidified water at pH 4 and 5 lowered ileum and caecum pH (p < 0.05) compared to pH 3. Acidified water at pH 4 showed the highest (p < 0.05) pepsin activity compared to pH 3 and pH 5. Considering the limited sample size and short-term assessment period of our screening test, the OAs with the highest potential for use in post-weaning rabbits were FOR, ACET, and CIT at pH 4. The selected combinations did not exhibit any early adverse effects in young rabbits. These results should be further confirmed in a broader population of animals. It would also be advisable to extend the application of OAs over longer periods to evaluate their effects throughout the entire growing period of rabbits.
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  • 文章类型: Journal Article
    印度枣(Ber)是高度易腐的更年期水果,原因是高腐烂指数限制了其适销性,并需要采取干预措施来延长保质期。杀菌剂通常用于在采后储存期间控制腐烂,然而,留下的残留物需要生态安全的替代品,如热水浸渍。成熟,将更年期前的枣浸入45、50或55°C的水中8、6或4分钟,然后储存在5℃进行定期质量评估。将水果浸入55°C的水中可使体重减轻32.69和35.27、64.21和58.57、30.41和30.42、38.50和52.20%,衰减指数,丙二醛(MDA)和电解质泄漏,与25°C的水浸相比,Umran和Pakwhite的抗氧化活性和抗坏血酸分别提高了15.40和16.77、19.51和20.48%。最高的葡萄糖,果糖,苹果酸,柠檬酸,酒石酸分别为23.44±1.04和29.9±0.95,30.68±1.72和41.17±2.34mg/100g,138.1±6.45和112.97±6.16、57.49±1.71和53.78±1.90、79.58±5.1和65.3±4.83μg/100g,而较低的蔗糖分别为12.34±0.94和16.33±1.05mg/100g。高浸水温度(55°C)表现出更好的质量与最低的衰变指数和重量损失,更大的膜完整性,生物活性物质含量和感官接受度评分。因此,热水浸渍被证明是一种有效的无残留选择,可以延长枣子的适销期以占领遥远的市场。
    Indian jujube (Ber) is highly perishable climacteric fruit owing to high decay index limiting its marketability and demands interventions to prolong shelf life. Fungicides are normally used to control rot during postharvest storage, however, residues left necessitate eco-safe alternatives like hot water dipping. Mature, pre-climacteric jujubes were dipped in 45, 50 or 55 °C water for 8, 6 or 4 min, respectively and then stored at 5 °C for periodic quality evaluation. Dipping fruits in 55 °C water resulted in 32.69 and 35.27, 64.21 and 58.57, 30.41 and 30.42, 38.50 and 52.20 % lower weight loss, decay index, malondialdehyde (MDA) and electrolyte leakage, whereas 15.40 and 16.77, 19.51 and 20.48 % greater antioxidant activity and ascorbic acid respectively for Umran and Pakwhite compared to 25 °C water dip. The highest glucose, fructose, malic, citric, and tartaric acids were 23.44 ± 1.04 and 29.9 ± 0.95, 30.68 ± 1.72 and 41.17 ± 2.34 mg/100 g, 138.1 ± 6.45 and 112.97 ± 6.16, 57.49 ± 1.71 and 53.78 ± 1.90, 79.58 ± 5.1 and 65.3 ± 4.83 μg/100 g whereas lower sucrose 12.34 ± 0.94 and 16.33 ± 1.05 mg/100 g were respectively recorded in 55 °C water dipped Umran and Pakwhite fruits. High dip water temperature (55 °C) exhibited better quality with the lowest decay index and weight loss, greater membrane integrity, bioactives content and sensory acceptance scores. Hence, hot water dipping was shown to be an effective residue-free option to extend the marketable period of jujubes to capture distant markets.
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  • 文章类型: Journal Article
    本研究旨在阐明贮藏温度对各种水果品质属性的影响。生理紊乱,以及在0.5、3或10°C储存的耐寒猕猴桃中的相关代谢物。剥蚀,在储存的0.5°C水果中最高,被确定为耐寒猕猴桃的冷害症状。脯氨酸和支链氨基酸含量在0.5°C储存的水果中显示出较高的值作为冷却响应。另一方面,储存5周后,果实的枯萎和腐烂在10°C最高。10℃贮藏诱导果实成熟3周,但是水果在储存5周后萎缩和腐烂严重。因此,在适当的温度下储存“秋感”耐寒猕猴桃会更有益,因为它改变了目标代谢物,并有助于减少冷藏过程中生理疾病的发生率。
    This study aimed to elucidate the effects of storage temperature on various fruit quality attributes, physiological disorders, and associated metabolites in the 0.5, 3, or 10 °C stored hardy kiwifruit. Peel pitting, which was highest in the 0.5 °C stored fruit, was identified as a chilling injury symptom of hardy kiwifruit. Proline and branched-chain amino acid contents showed higher values at 0.5 °C stored fruit as chilling responses. On the other hand, fruit shriveling and decay were highest in the 10 °C after 5 weeks of storage. The 10 °C storage induced fruit ripening during 3 weeks, but fruit shriveling and decay were severe after 5 weeks of storage. Therefore, storing the \'Autumn Sense\' hardy kiwifruit at proper temperatures would be more beneficial, as it alters targeted metabolites and helps reduce the incidence of physiological disorders during cold storage.
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  • 文章类型: Journal Article
    在家禽饲料中过度使用抗生素会带来灾难性的影响;因此,必须开发长期替代品。因此,本研究旨在评估黑曲霉滤液(ANF)中有机酸含量高的蚕豆(AIRFB)对鹌鹑饮食的影响,以及它们对鸟类生产力的影响,消化,car体产量,血液化学,和肠道微生物群。本研究共使用240只日本鹌鹑(7日龄),平均分为5个实验组,每组48只鹌鹑。第1组(G1)接受无任何ANF的基础饮食,第2组(G2)接受补充0.5mLANF/kg饮食的基础饮食,第3组(G3)接受补充1.0mLANF/kg饮食的基础饮食,第4组(G4)接受补充1.5mLANF/kg饮食的基础饮食,第5组(G5)接受补充2mLANF/kg饮食的基础饮食。在1至3周、3至5周和1至5周监测性能参数。添加ANF在3周和5周时增加了体重,以及在1至3周、3至5周和1至5周体重增加,与对照饮食相比。与对照组相比,饲喂ANF的鹌鹑的饲料转化率最高。鹌鹑饮食中添加ANF对car体的重量没有影响,Gizzard,心,肝脏,内脏,或敷料;然而,它确实降低了甘油三酯,低密度脂蛋白,和极低密度脂蛋白,同时增加高密度脂蛋白水平。接受ANF的鹌鹑组的免疫学指标增强,例如IgG,IgM,IgA,和溶菌酶.它还增加了超氧化物歧化酶和总抗氧化剂含量的水平,以及过氧化氢酶,和消化酶,如蛋白酶,淀粉酶,和脂肪酶。然而,与对照组相比,它降低了血液MDA水平。已经证明,总的肠道微生物群,大肠杆菌,总大肠菌群,ANF喂养的鹌鹑中沙门氏菌的数量都减少了。ANF鹌鹑肝脏和肠道切片的组织学检查显示肝实质正常,典型的叶状肠绒毛,和相对短且经常自由的发光。总之,日本鹌鹑的表现有所改善,消化酶,抗氧化剂指数,豁免权,以及食用补充ANF的饮食后减少肠道致病菌的能力。
    Antibiotic overuse in poultry feeds has disastrous implications; consequently, long-term alternatives must be developed. As a result, the current study aims to assess the impact of Aspergillus niger filtrate (ANF) high in organic acids grown on agro-industrial residue of faba bean (AIRFB) on quail diet, as well as their influence on bird productivity, digestion, carcass yield, blood chemistry, and intestinal microbiota. A total of 240 Japanese quails (aged 7 d) were used in this study, divided equally among 5 experimental groups with 48 quails each. Group 1 (G1) received a basal diet without any ANF, group 2 (G2) received a basal diet supplemented with 0.5 mL ANF/kg diet, group 3 (G3) received a basal diet supplemented with 1.0 mL ANF/kg diet, group 4 (G4) received a basal diet supplemented with 1.5 mL ANF/kg diet, and group 5 (G5) received a basal diet supplemented with 2 mL ANF/kg diet. The performance parameters were monitored at 1 to 3, 3 to 5, and 1 to 5 wk. Adding ANF increased body weight at 3 and 5 wk, as well as body weight gain at 1 to 3, 3 to 5, and 1 to 5 wk, compared to the control diet. The ANF fed quails had the highest feed conversion ratio compared to the control group. The addition of ANF to the quail diet had no effect on the weight of the carcass, gizzard, heart, liver, giblets, or dressing; however, it did lower triglycerides, low-density lipoprotein, and very low-density lipoprotein while increasing high-density lipoprotein levels. The quail groups that received ANF had enhanced immunological indices such as IgG, IgM, IgA, and lysozymes. It also increased the levels of superoxide dismutase and total antioxidant contents, as well as catalase, and digestive enzymes such as protease, amylase, and lipase. However, it lowered the blood MDA levels compared to control. It has been demonstrated that the total gut microbiota, Escherichia coli, total coliforms, and the population of Salmonella are all reduced in ANF-fed quails. Histological examination of ANF quails\' liver and intestinal sections revealed normal hepatic parenchyma, typical leaf-like intestinal villi, and comparatively short and frequently free lumina. In conclusion, Japanese quail showed improvements in performance, digestive enzymes, antioxidant indices, immunity, and capacity to reduce intestinal pathogenic bacteria after consuming diet supplemented with ANF.
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  • 文章类型: Journal Article
    这项研究探索了通过使用黑曲霉优化草酸生产,通过固态发酵(SSF)来增强磷矿(PR)溶解。关键工艺参数,包括使用农业工业副产品(甘蔗渣(SCB),麦麸(WB),大豆麸皮(SB)),pH值,蔗糖补充,和甲醇添加,通过序贯实验设计进行了系统评估。结果确定比例为1:1的SCB和SB是最有效的底物。值得注意的是,包含甲醇(7%)和蔗糖(0.5%)导致草酸产量增加3倍。在这些优化条件下,显著的磷溶解的Bayóvar,Itafos,并且实现了注册PR,Bayóvar岩石释放高达12.1g/kgds的可溶性磷(效率为63.8%)。此外,SSF工艺有效地从农用工业基质中释放有机磷。这些发现为推进生物经济和开发未来的工业生物肥料提供了希望。
    This study explores the enhancement of phosphate rock (PR) solubilization through solid-state fermentation (SSF) by optimizing oxalic acid production using Aspergillus niger. Key process parameters, including the use of agro-industrial by-products (sugarcane bagasse (SCB), wheat bran (WB), soybean bran (SB)), pH levels, sucrose supplementation, and methanol addition, were systematically evaluated through sequential experimental designs. The results identified SCB and SB in a 1:1 ratio as the most effective substrate. Remarkably, the inclusion of methanol (7 %) and sucrose (0.5 %) resulted in a 3-fold increase in oxalic acid production. Under these optimized conditions, significant phosphorus solubilization of Bayóvar, Itafós, and Registro PRs was achieved, with Bayóvar rock releasing up to 12.1 g/kgds of soluble P (63.8 % efficiency). Additionally, the SSF process effectively released organic phosphorus from the agro-industrial substrates. These findings hold promise for advancing the bio-based economy and developing future industrial biofertilizers.
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  • 文章类型: Journal Article
    每年,全球产生约20亿吨植物源性废物(如秸秆和作物残留物),其中大部分要么被焚化,甩了,或在没有适当规划的情况下填埋,导致严重的环境污染和资源浪费。植物来源的废物在各个方面表现出作为生长介质组分的潜在优势。然而,大量研究还表明,植物来源的废物通常具有很强的植物毒性,在用作生长培养基成分之前必须去除或减少。因此,准确地评估它们的植物毒性并适当地修改它以确保它们在用作生长培养基成分时支持植物生长是至关重要的。本文综述了植物源性废物中植物毒性的表现和评估方法;系统地总结了三种常见的植物源性废物的植物毒性来源(花园废物,农作物秸秆,和用过的蘑菇基质),以及两种具有代表性的植物毒性物质(酚类化合物和有机酸)的毒性机理;并对进一步的研究方向提出了一些见解。通过巩固这些研究的见解,这篇综述旨在加深我们对植物毒性及其影响的理解,为今后的研究工作和实际应用提供有价值的参考和指导。
    Every year, approximately 2 billion tons of plant-derived waste (such as straw and crop residues) are generated globally, most of which are either incinerated, dumped, or landfilled without proper planning, leading to severe environmental pollution and resource wastage. Plant-derived waste exhibits potential advantages as a growing media component in various aspects. However, numerous studies have also indicated that plant-derived waste generally possesses strong phytotoxicity, which must be removed or reduced before being utilized as a growing media component. Therefore, accurately assessing their phytotoxicity and appropriately modifying it to ensure their support for plant growth when used as a growing media component is crucial. This paper reviews the manifestation and assessment methods of phytotoxicity in plant-derived waste; systematically summarizes the phytotoxicity sources of three common types of plant-derived waste (garden waste, crop straw, and spent mushroom substrate), as well as the toxic mechanisms of two representative phytotoxic substances (phenolic compounds and organic acids); and proposes some insights into further research directions. By consolidating insights from these studies, this review aims to deepen our understanding of phytotoxicity and its implications, and offer valuable references and guidance for future research endeavors and practical applications.
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  • 文章类型: Journal Article
    阻止马铃薯汁在食品工业中应用的因素之一是其酶促褐变的倾向。添加富含有机酸的水果和蔬菜浓缩物可以减少马铃薯汁颜色的不利变化并影响生物活性化合物的含量。本研究旨在研究向马铃薯汁中添加天然水果和蔬菜浓缩物对其颜色以及从中分离出的花色苷色素的组成和含量的影响。颜色,用HPLC-DAD和UHPLCMS/MS液相色谱法测定马铃薯汁和染料中花色苷的组成和含量。发现没有添加浓缩物的果汁,平均而言,与添加浓缩物的果汁相比,总多酚含量降低61%,花色苷含量降低63%。所包含的孤立颜料,平均而言,与从未纯化的果汁中分离的花色苷相比,花色苷多30-40倍。此外,浓缩物的添加使大约。与没有这些添加剂的样品相比,花青素的有效分离提高了3-5倍。添加浓缩物的马铃薯汁和染料显示出较浅的颜色,不会随时间变化,与没有浓缩物的样品相比。
    One of the factors precluding potato juice application in the food industry is its propensity for enzymatic browning. The addition of fruit and vegetable concentrates rich in organic acids can reduce unfavorable changes in the color of potato juices and influence the content of biologically active compounds. This study aimed to investigate the effect of the addition of natural fruit and vegetable concentrates to potato juices on their color and on the composition and contents of anthocyanin pigments isolated from them. The color, composition and amount of anthocyanins in potato juices and dyes were determined using HPLC-DAD and UHPLC MS/MS liquid chromatography. It was found that the juices without concentrate addition had, on average, 61% lower total polyphenol content and 63% lower anthocyanin content compared with the juices with added concentrates. The isolated pigments contained, on average, 30-40 times more anthocyanins compared with those isolated from the non-purified juices. Furthermore, the addition of concentrates enabled ca. 3-5 times more effective isolation of anthocyanins compared with the samples without these additives. Potato juices and dyes with the addition of concentrates showed a lighter color that did not change over time, compared with samples without concentrates.
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  • 文章类型: Journal Article
    研究了固液提取以获得选定的主要植物营养素(P,K,Ca,Mg)使用水杨酸等弱有机酸从生物燃料灰分中提取,柠檬酸,和草酸作为牺牲浸出剂。在这项研究中,比较了三种有机酸,以确定最有效的浸出剂,以最大化P,K,Ca,从生物燃料灰中提取镁。结果表明,0.1M柠檬酸对植物养分回收最有效,30分钟后P恢复81.9%,Ca的82.4%,镁的76.8%,120分钟后K的47.3%。最高的K,用0.1M草酸提取180分钟后回收59.3%。然而,P-80.7%的回收率较低,Ca-2.3%的回收率低得多,用0.1M草酸提取180分钟后,Mg-68.6%。渗滤液没有被重金属污染,只有0.47毫克/升的锌,7.67mg/L的铝,用0.1M草酸提取180分钟后检测到1.99mg/L的铁。通过SEM-EDS观察到用0.1M草酸萃取后草酸钙的形成。研究结果表明,要实现所有有益营养素的最高回收率(P,K,Ca,Mg)需要不同的提取次数和不同的提取剂。
    Solid-liquid extraction was investigated to obtain selected major plant nutrients (P, K, Ca, Mg) from biofuel ash using weak organic acids like salicylic acid, citric acid, and oxalic acid as sacrificial leaching agents. In this study, three organic acids were compared to determine the most effective leaching agent for maximizing the P, K, Ca, and Mg extraction from biofuel ash. The findings indicated that 0.1 M citric acid was the most efficient for plant nutrient recovery, with 81.9% of P recovered after 30 min, 82.4% of Ca, 76.8% of Mg, and 47.3% of K. after 120 min. The highest amount of K, with 59.3% was recovered after 180 min of extraction with 0.1 M oxalic acid. However, recovery of P-80.7% was lower, and much lower recovery of Ca-2.3%, and Mg-68.6% after 180 min of extraction with 0.1 M oxalic acid. The leachates were not contaminated with heavy metals, just 0.47 mg/L of Zn, 7.67 mg/L of Al, and 1.99 mg/L of Fe were detected after 180 min of extraction with 0.1 M oxalic acid. The formation of calcium oxalates after extraction with 0.1 M oxalic acid was seen by SEM-EDS. The findings indicated that to achieve the highest recovery of all beneficial nutrients (P, K, Ca, Mg) different extraction times and different extraction agents are required.
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