肠道类器官已成为研究肠道复杂结构和功能的强大模型系统。然而,缺乏广泛适用的收集方法,标签,和肠道类器官的成像。在这项研究中,我们开发了一种新的装载和标记肠道类器官的方法,一种有效收集类器官并促进其三维(3D)结构成像的方法。基于这个过滤器平台,小鼠肠道器官被充分收集和固定,促进对类器官的靶蛋白的免疫标记工作流程。经过评估,40μm的过滤器被认为更有利于小鼠肠道类器官的收集和标记。对多种类型和物种起源的类器官的更广泛研究将有助于扩大该方法的适用性。总的来说,我们的研究提出了加载和分析肠道类器官的创新工作流程.结合基于过滤器的收集方法,荧光标记,三维重建提供了对这些组织模型的组织和复杂性的宝贵见解,从而为研究肠道发育提供了新的途径,疾病建模,和药物发现。
Intestinal organoids have emerged as powerful model systems for studying the complex structure and function of the intestine. However, there is a lack of widely applicable methods for the collection,
labeling, and imaging of intestinal organoids. In this study, we developed a novel method for loading and
labeling intestinal organoids, a method that efficiently collects the organoids and facilitates imaging of their three-dimensional (3D) structure. Based on this strainer platform, mouse intestinal organoids were adequately collected and immobilized, facilitating the immunolabeling workflow to target proteins of the organoids. After evaluation, the strainer size of 40 μm was considered to be more conducive to the collection and
labeling of mouse intestinal organoids. More extensive research on organoids of multiple types and species origins will contribute to broadening the applicability of the methodology. Overall, our study proposes an innovative workflow for loading and analyzing intestinal organoids. The combination of a strainer-based collection method, fluorescent
labeling, and 3D reconstruction provides valuable insights into the organization and complexity of these tissue models, thereby offering new avenues for investigating intestinal development, disease modeling, and drug discovery.