Abstract:
Prenylation (isopentenylation), a key post-transcriptional modification with a hydrophobic prenyl group onto the biomacromolecules such as RNA and proteins, influences their localization and function. Prenyltransferases mediate this process, while cytokinin oxidases degrade the prenylated adenosine in plants. This review summarizes current progress in detecting prenylation modifications in RNA across species and their effects on protein synthesis. Advanced methods have been developed to label and study these modifications in vitro and in vivo, despite challenges posed by the inert chemical properties of prenyl groups. Continued advancements in bioorthogonal chemistry promise new tools for understanding the precise biological functions of prenylated RNA modifications and other related proteins.
摘要:
丙炔化(异戊烯化),在RNA和蛋白质等生物大分子上使用疏水性异戊二烯基进行关键的转录后修饰,影响它们的定位和功能。脯氨酸转移酶介导了这个过程,而细胞分裂素氧化酶降解植物中的异戊二烯化腺苷。本文综述了跨物种RNA中异戊二烯化修饰的检测及其对蛋白质合成影响的研究进展。已经开发了先进的方法来在体外和体内标记和研究这些修饰,尽管异戊二烯基的惰性化学性质带来了挑战。生物正交化学的不断进步为理解异戊二烯化RNA修饰和其他相关蛋白质的精确生物学功能提供了新工具。