目的:血管周围脂肪组织(PVAT)分泌的硫化氢(H2S)是一种关键的血管扩张剂,这可能与高血压的发病机制有关。本研究旨在探讨H2S在肥胖高血压患者长期运动调节PVAT抗收缩中的确切作用。
方法:通过高脂饮食建立肥胖高血压(24周)后,雄性SD大鼠随机分为对照组(HC),运动组(HE),胱硫醚γ-裂解酶(CSE)阻断组(HCB),运动结合CSE阻断组(HEB)。在整个13周内进行运动和CSE抑制剂方案。
结果:经过13周的干预,长期运动显著降低了血压(HCvs.他,P<0.05),但不是通过运动结合CSE抑制剂方案。同时,即使在运动后,CSE抑制剂也能显著阻断PVAT中H2S的产生(HE与HEB,P<0.05)。此外,长期运动改变了电压依赖性K+(Kv)通道亚基7(KCNQs)的表达,肠系膜动脉的CSE抑制作用减弱。至于血管张力评估,在有或没有KCNQ开放剂(瑞替加宾)的情况下孵育后,PVAT(有或没有转移的PVAT浴液)的抗收缩作用通过长期运动显着增强,并通过CSE抑制剂方案消除(P<0.05);除HE外,KCNQ抑制剂(XE991)减弱了这种作用。
结论:这些结果共同表明,内源性H2S是长期运动对肥胖高血压患者PVAT的抗收缩作用的强调节剂,在此过程中,阻力动脉中的KCNQ可能参与其中,但不是H2S介导的唯一靶通道。
OBJECTIVE: Hydrogen sulfide (H2S) secreted by perivascular adipose tissue (PVAT) is a critical vasodilator, which might be involved during the pathogenesis of hypertension. The present study aimed to investigate the exact role of H2S on the regulation of PVAT anti-contraction by long-term exercise in obesity hypertension.
METHODS: After the establishment of obesity hypertension (24 weeks) through a high-fat diet, male Sprague-Dawley rats were randomly assigned to control group (HC), exercise group (HE), cystathionine γ-lyase (CSE) blocking group (HCB), and exercise combined with CSE blocking group (HEB). Exercise and CSE inhibitor regimens were performed throughout 13 weeks.
RESULTS: After 13 weeks of intervention, blood pressure was significantly decreased by long-term exercise (HC vs. HE, P < 0.05) but not by exercise combined with the CSE inhibitor regimen. Meanwhile, the CSE inhibitor significantly blocked the production of H2S in PVAT even after exercise (HE vs. HEB, P < 0.05). Furthermore, long-term exercise altered the expressions of voltage-dependent K+ (Kv) channel subunits 7 (KCNQs), which were diminished by CSE inhibition in mesenteric arteries. As for vascular tension assessment, after incubation with or without KCNQ opener (retigabine), the anti-contractile effect of PVAT (with or without transferred bath solution of PVAT) was significantly enhanced by long-term exercise and eliminated by the CSE inhibitor regimen (P < 0.05); KCNQ inhibitor (XE991) blunted this effect except for HE.
CONCLUSIONS: These results collectively suggest that endogenous H2S is a strong regulator of the anti-contractile effect of PVAT in obesity hypertension by long-term exercise, and KCNQ in the resistance artery might be involved during this process but not the only target channel mediated by H2S.