■半胱硫醚β-合酶(CBS)和半胱硫醚γ-裂解酶(CSE)是参与一碳代谢的两种重要酶。这些酶通过参与反式硫化途径中谷胱甘肽(GSH)抗氧化剂的合成,在调节男性因素不育的氧化应激和炎症中起重要作用。此外,硫化氢(H2S)的直接释放具有抗炎和抗氧化作用。因此,对不育和精索静脉曲张男性中CBS和CSE基因mRNA水平的表达进行了评估,并与健康者进行了比较,以阐明其在男性不育病理中的可能作用.
■在本病例对照研究中,精液参数评估(浓度,形态学,和精子的运动性)在28例精索静脉曲张患者上进行,43名精子参数异常的不育男性,和19个肥沃的男人。从精子样本中提取RNA,然后使用CBS进行cDNA合成和实时聚合酶链反应(PCR),CSE,和GAPDH引物。
■不育和精索静脉曲张组的精子浓度和运动性均显着降低(P=0.001),而精子正常形态高于可育组(P=0.05)。不育组(P=0.04和P=0.037)和精索静脉曲张组(P=0.01和P=0.046)中CBS和CSE基因的表达水平均显著低于可育组。此外,CBS基因表达与精子参数呈正相关(r=0.296,P=0.025)。
■根据我们的发现,考虑CBS和CSE酶在男性因素不育症中的主要作用有一个合理的理由,这可能特别指出必需抗氧化剂(包括H2S)的释放不足是不育症的分子基础,值得进一步研究.
UNASSIGNED: Cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE) are two important enzymes involved in One-Carbon metabolism. These enzymes play important roles in modulating oxidative stress and inflammation in male factor infertility through participating in the synthesis of glutathione (GSH) antioxidants in the trans-sulfuration pathway. Besides, the direct release of hydrogen sulfide (H2S) has anti-inflammatory and antioxidant effects. Therefore, the expression of CBS and CSE genes at mRNA levels in infertile and varicocele men was evaluated and compared to the healthy counterparts to clarify their possible role in the pathology of male infertility.
UNASSIGNED: In this
case-control study, semen parameter assessment (concentration, morphology, and motility of sperms) was performed on 28 men with varicocele, 43 infertile men with abnormal sperm parameters, and 19 fertile men. RNA was extracted from sperm samples followed by cDNA synthesis and real-time polymerase chain reaction (PCR) using CBS, CSE, and GAPDH primers.
UNASSIGNED: Sperm concentration and motility in infertile and varicocele groups were significantly lower (P=0.001), while spermatoza normal morphology was higher than fertile group (P=0.05). The expression levels of both CBS and CSE genes in infertile (P=0.04 and P=0.037 respectively) and varicocele (P=0.01 and P=0.046 respectively) groups were significantly lower than fertile group. Additionally, CBS gene expression indicated a positive correlation with expression of CBS gene (r=0.296, P=0.025) and sperm parameters.
UNASSIGNED: In light of our findings, there is a valid rationale to consider the primary role of CBS and CSE enzymes impairment in male factor infertility which specifically may point to a deficit in the release of essential antioxidants including the H2S as a molecular basis of infertility and warrants further investigation.