Abstract：
It has been well established that Hydrogen sulfide (H2S) is involved in various pathophysiological processes. Therefore, accurate monitoring H2S levels in vitro or vivo is of great significance in biological systems. Herein, we firstly developed a thiomaleimide-based compound MAL-1 bearing aggregation-induced emission characteristic for selective response toward H2S due to its nucleophilicity. The proposed sensor presented prominent sensitivity and selectivity with low detection limit of 75 nM and pseudo-first-order reaction rate constant of 9.65 × 10-2 s-1, as well as low cytotoxicity which works well in recognizing H2S in real samples and visualizing H2S in living cells. Thus, it could be concluded that the novel thiomaleimide-based probe would be a promising tool for assessing intracellular H2S levels.
摘要：
已经确定硫化氢(H2S)参与各种病理生理过程。因此,在体外或体内准确监测H2S水平在生物系统中具有重要意义。在这里，我们首先开发了基于硫代马来酰亚胺的化合物MAL-1，该化合物具有聚集诱导的发射特性，由于其亲核性，因此具有对H2S的选择性响应。所提出的传感器具有突出的灵敏度和选择性，具有75nM的低检测限和9.65×10-2s-1的伪一级反应速率常数，以及低细胞毒性，可以很好地识别真实样品中的H2S并可视化活细胞中的H2S。因此，可以得出结论，新型的基于硫代马来酰亚胺的探针将是评估细胞内H2S水平的有前途的工具。
