干旱已成为影响植物生长和产量的严重环境因子。Fructan,作为大蒜中重要的储存化合物,在耐旱性中起着重要作用。干旱胁迫下植物的基因组变化阐明了植物响应胁迫的分子机制。因此,我们使用RNA-seq来确定干旱胁迫下大蒜的转录组变化,并通过加权基因共表达网络分析确定了与果聚糖代谢相关的关键模块。我们对干旱胁迫下一段时间(0、3、6、9、12、15d)的大蒜转录组进行了综合分析。干旱显著诱导基因表达的变化。特异性表达基因的数量为1430(3d),399(6d),313(9d),351(12d),和1882年(15天),每个时间点只有114个基因做出反应。上调的DEGs的数量高于下调的DEGs的数量。基因本体论和京都基因和基因组百科全书分析表明,在干旱胁迫下,大蒜更容易引起碳水化合物代谢途径的变化。果聚糖含量测定表明,干旱胁迫显著诱导大蒜果聚糖积累。为了确定大蒜中果聚糖含量的转录调控是否涉及模块,我们使用WGCNA进一步分析了与果聚糖代谢相关的基因。它们被丰富了两个模块,以F-box蛋白和GADPH为hub基因,参与响应干旱胁迫的大蒜果聚糖代谢。这些研究结果为今后研究和培育耐旱大蒜品种提供了重要启示。
Drought has become a serious environmental factor that affects the growth and yield of plants.
Fructan, as an important storage compound in garlic, plays an important role in drought tolerance. Genomic changes in plants under drought stress clarify the molecular mechanism of plants\' responses to stress. Therefore, we used RNA-seq to determine the transcriptomic changes in garlic under drought stress and identified the key module related to
fructan metabolism by weighted gene co-expression network analysis. We conducted a comprehensive analysis of the garlic transcriptome under drought stress over a time course (0, 3, 6, 9, 12, 15 d). Drought significantly induces changes in gene expression. The number of specifically expressed genes were 1430 (3 d), 399 (6 d), 313 (9 d), 351 (12 d), and 1882 (15 d), and only 114 genes responded at each time point. The number of upregulated DEGs was higher than the number of downregulated DEGs. Gene ontology and a Kyoto Encyclopedia of Genes and Genomes analysis showed that garlic was more likely to cause changes in carbohydrate metabolism pathways under drought stress.
Fructan content measurements showed that drought stress significantly induced
fructan accumulation in garlic. To determine whether there were modules involved in the transcriptional regulation of
fructan content in garlic, we further analyzed the genes related to
fructan metabolism using WGCNA. They were enriched in two modules, with F-box protein and GADPH as hub genes, which are involved in garlic fructan metabolism in response to drought stress. These results provide important insights for the future research and cultivation of drought-tolerant garlic varieties.