Abstract：
Ophiopogonis Radix is a well-known Traditional Chinese Medicine and functional food that is rich in polysaccharides and has fructan as a characteristic component. In this study, an inulin neoseries-type fructan designated as OJP-W2 was obtained and characterized from Ophiopogonis Radix, and its potential therapeutic effect on liver fibrosis in vivo were investigated. Structural studies revealed that OJP-W2 had a molecular weight of 5.76 kDa and was composed of glucose and fructose with a molar ratio of 1.00:30.87. Further analysis revealed OJP-W2 has a predominantly lineal (1-2)-linked β-D-fructosyl units linked to the glucose moiety of the sucrose molecule with (2-6)-linked β-D-fructosyl side chains. Pharmacological studies revealed that OJP-W2 exerted a marked hepatoprotective effect against liver fibrosis, the mechanism of action was involved in regulating collagen deposition (α-SMA, COL1A1 and liver Hyp contents) and TGF-β/Smads signaling pathway, alleviating liver inflammation (IL-1β, IL-6, CCL5 and F4/80) and MAPK signaling pathway, and inhibiting hepatic apoptosis (Bax, Bcl-2, ATF4 and Caspase 3). These data provide evidence for expanding Ophiopogonis Radix-acquired fructan types and advancing our understanding of the specific role of inulin neoseries-type fructan in liver fibrosis therapy.
摘要：
麦冬是一种著名的中药和功能性食品，富含多糖，以果聚糖为特征成分。在这项研究中,从麦冬获得并表征了一种名为OJP-W2的菊粉新系列果聚糖，并研究了其对体内肝纤维化的潜在治疗作用。结构研究表明，OJP-W2的分子量为5.76kDa，由葡萄糖和果糖组成，摩尔比为1.00：30.87。进一步分析显示,OJP-W2具有主要的线性(1-2)-连接的β-D-果糖基单元,其与具有(2-6)-连接的β-D-果糖基侧链的蔗糖分子的葡萄糖部分连接。药理学研究表明，OJP-W2对肝纤维化具有明显的肝保护作用，作用机制涉及调节胶原沉积(α-SMA，COL1A1和肝脏Hyp含量）和TGF-β/Smads信号通路，缓解肝脏炎症(IL-1β，IL-6，CCL5和F4/80）和MAPK信号通路，并抑制肝细胞凋亡(Bax，Bcl-2，ATF4和Caspase3）。这些数据为扩大麦冬获得的果聚糖类型和促进我们对菊粉新系列果聚糖在肝纤维化治疗中的具体作用的理解提供了证据。
