Abstract：
Allium Macrostemon Bge. (AMB) is a well-known homology of herbal medicine and food that has been extensively used for thousands of years to alleviate cardiovascular diseases. It contains a significant amount of polysaccharides, yet limited research exists on whether these polysaccharides are responsible for its cardiovascular protective effects. In this study, the anti-atherosclerosis effect of the crude polysaccharides of AMB (AMBP) was evaluated using ApoE-/- mice fed a high-fat diet, along with ox-LDL-induced Thp-1 foam cells. Subsequently, guided by the inhibitory activity of foam cells formation, a major homogeneous polysaccharide named AMBP80-1a was isolated and purified, yielding 11.1 % from AMB. The molecular weight of AMBP80-1a was determined to be 10.01 kDa. AMBP80-1a was firstly characterized as an agavin-type fructan with main chains consisting of →1)-β-d-Fruf-(2→ and →1,6)-β-d-Fruf-(2→ linked to an internal glucose moiety, with →6)-β-d-Fruf-(2→ and β-d-Fruf-(2→ serving as side chains. Furthermore, the bio-activity results indicated that AMBP80-1a reduced lipid accumulation and cholesterol contents in ox-LDL-induced Thp-1 foam cell. These findings supported the role of AMBP in alleviating atherosclerosis in vivo/vitro. AMBP80-1a, as the predominant homogeneous polysaccharide in AMB, was expected to be developed as a functional agent to prevent atherosclerosis.
摘要：
葱葱MacrostemonBge.（AMB）是众所周知的草药和食品的同源性，已被广泛使用了数千年来缓解心血管疾病。它含有大量的多糖，然而，关于这些多糖是否负责其心血管保护作用的研究有限。在这项研究中,AMB粗多糖（AMBP）的抗动脉粥样硬化作用是使用高脂饮食喂养的ApoE-/-小鼠进行评估，以及ox-LDL诱导的Thp-1泡沫细胞。随后，以泡沫细胞形成的抑制活性为导向，分离并纯化了一种名为AMBP80-1a的主要均质多糖，来自AMB的收益率为11.1%。AMBP80-1a的分子量测定为10.01kDa。AMBP80-1a首先被表征为龙舌兰型果聚糖，其主链由→1）-β-d-Fruf-（2→和→1,6）-β-d-Fruf-（2→连接到内部葡萄糖部分，与→6）-β-d-Fruf-（2→和β-d-Fruf-（2→作为侧链。此外，生物活性结果表明，AMBP80-1a降低了ox-LDL诱导的Thp-1泡沫细胞的脂质积累和胆固醇含量。这些发现支持AMBP在体内/体外缓解动脉粥样硬化中的作用。AMBP80-1a,作为AMB中主要的均质多糖，有望被开发为预防动脉粥样硬化的功能剂。
