本研究旨在基于代谢差异揭示不同品种白花前胡花的品质形成,为该药材的开发利用提供理论依据。对六个品种(YS,H,LZ,LY,LX,和Z)具有相同的起源和相同的发育阶段。主成分分析,正交偏最小二乘判别分析,并进行单因素统计分析,筛选不同品种的差异代谢产物。基于质荷比预测与质量形成相关的潜在生物标志物,京都基因和基因组途径富集百科全书,相关文献资料,和相关分析。结果表明,6个品种的代谢物差异显著,在正离子和负离子模式下获得571和465种差异代谢物,分别。从差异代谢物中,预测了22种与质量形成相关的潜在生物标志物,涉及9种代谢途径,包括苯丙氨酸,酪氨酸和色氨酸的生物合成,苯丙素的生物合成,和植物激素的生物合成。与YS品种相比,其他品种显示补骨脂素浓度降低,欧周素,和姜黄素和增加浓度的7-羟基香豆素,esculetin,columbianetin,还有茉莉酸,参与苯丙素的生物合成。2-琥珀酰苯甲酸酯的浓度,Heraclenol,和L-酪氨酸参与其他代谢途径减少,特别是在Z和H品种中。因此,调节苯丙素的生物合成是提高白花假单胞菌品种品质的关键机制之一。Z和H品种比其他品种具有更好的品质和代谢过程,因此可用于优质种质的筛选和育种。
This study aims to reveal the quality formation of different
cultivars of Peucedanum praeruptorum based on the metabolic differences and provide a theoretical basis for the development and utilization of this medicinal herb. The non-target metabonomics analysis based on ultra-high performance liquid chromatography tandem mass spectrometry(UHPLC-MS/MS) was conducted for six
cultivars(YS, H, LZ, LY, LX, and Z) of P. praeruptorum of the same origin and at the same development stage. The principal component analysis, orthogonal partial least squares discriminant analysis, and univariate statistical analysis were carried out to screen the differential metabolites of different
cultivars. The potential biomarkers associated with quality formation were predicted based on the mass-to-charge ratio, Kyoto Encyclopedia of Genes and Genomes pathway enrichment, information of relevant literature, and correlation analysis. The results showed that metabolites differed significantly among the six cultivars, and 571 and 465 differential metabolites were obtained in the positive and negative ion modes, respectively. From the differential metabolites, 22 potential biomarkers related to quality formation were predicted, which involved 9 metabolic pathways, including phenylalanine, tyrosine and tryptophan biosynthesis, biosynthesis of phenylpropanoids, and biosynthesis of plant hormones. Compared with the YS cultivar, other
cultivars showed decreased concentrations of psoralen, imperatorin, and luvangetin and increased concentrations of 7-hydroxycoumarine, esculetin, columbianetin, and jasmonic acid, which were involved in the biosynthesis of phenylpropanoids. The concentrations of 2-succinylbenzoate, heraclenol, and L-tyrosine involved in other metabolic pathways decreased, especially in the Z and H cultivars. Therefore, regulating the biosynthesis of phenylpropanoids is one of the key mechanisms for improving the cultivar quality of P. praeruptorum. The Z and H
cultivars have better quality and metabolic processes than other
cultivars and thus can be used for the screening and breeding of high-quality germplasm.