PDF(Pubmed)
Abstract：
Alfalfa (Medicago sativa L.) is an important forage legume and soil salinization seriously affects its growth and yield. In a previous study, we identified a salt-tolerant variety \'Gongnong NO.1\' and a salt-sensitive variety \'Sibeide\'. To unravel the molecular mechanism involved in salt stress, we conducted transcriptomic analysis on these two cultivars grown under 0 and 250 mM NaCl treatments for 0, 12, and 24 h. Totals of 336, and 548 differentially expressed genes (DEGs) in response to NaCl were, respectively, identified in the \'Gongnong NO.1\' and \'Sibeide\' varieties. The Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) pathway enrichment analysis showed that the DEGs were classified in carbohydrate metabolism, energy production, transcription factor, and stress-associated pathway. Expression of MsHPCA1, encoding a putative H2O2 receptor, was responsive to both NaCl and H2O2 treatment. MsHPCA1 was localized in cell membrane and overexpression of MsHPCA1 in alfalfa increased salt tolerance and H2O2 content. This study will provide new gene resources for the improvement in salt tolerance in alfalfa and legume crops, which has important theoretical significance and potential application value.
摘要：
紫花苜蓿(MedicagosativaL.)是重要的豆科牧草,土壤盐渍化严重影响其生长和产量。在之前的研究中,我们确定了一个耐盐品种“工农1号”和一个盐敏感品种“西贝德”。为了揭示盐胁迫的分子机制，我们对这两个在0和250mMNaCl处理下生长的品种进行了转录组学分析，分别为0、12和24小时。响应NaCl的总共336和548个差异表达基因（DEGs）为，分别,在“工农1号”和“西贝德”品种中确定。京都基因和基因组百科全书（KEGG）和基因本体论（GO）途径富集分析表明，DEGs被分类为碳水化合物代谢，能源生产，转录因子，和应激相关途径。MsHPCA1的表达，编码推定的H2O2受体，对NaCl和H2O2处理都有反应。MsHPCA1位于细胞膜上，过表达MsHPCA1的苜蓿增加了耐盐性和H2O2含量。本研究将为提高苜蓿和豆科作物的耐盐性提供新的基因资源,具有重要的理论意义和潜在的应用价值。
