背景:抑郁症的定义是持续的情绪低落和睡眠模式的中断,世卫组织预测,到2030年,重度抑郁症将成为全球疾病负担的第三大主要贡献者。睡眠不足是一种应激源,会引发中枢神经系统内的炎症,一个被称为神经炎症的过程。这种炎症反应通过上调导致焦虑等症状的炎症介质的表达,在抑郁症的发展中起着至关重要的作用。绝望,失去快乐。
方法:在本研究中,睡眠剥夺被用作诱导小鼠焦虑和抑郁样行为的方法。然后使用EZM评估小鼠的行为变化,EPM,TST,FST,和SPT。H&E染色和Nissl染色用于检测内侧前额叶皮质(mPFC)区域的形态学变化。Elisa评估血清CORT水平。检测时钟基因的mRNA水平和蛋白质表达,高迁移率基因组盒-1(Hmgb1),静默消息调节器6(Sirt6),和促炎因子通过RT-qPCR,西方印迹,和免疫荧光技术。
结果:睡眠不足导致对陌生领域的探索减少,在绝望中度过的时间增加,降低小鼠蔗糖水的摄入量。此外,睡眠剥夺导致血清CORT分泌增加和时钟基因上调,IL6,IL1β,TNFα,Cox-2iNOS,Sirt6和Hmgb1。睡觉
结论:睡眠剥夺会导致大脑中的焦虑-抑郁样行为和神经炎症。时钟基因的转录和Sirt6/Hmgb1途径的激活可能有助于mPFC中的炎症反应。
BACKGROUND: Depression is defined by a persistent low mood and disruptions in sleep patterns, with the WHO forecasting that major depression will rank as the third most prevalent contributor to the global burden of disease by the year 2030. Sleep deprivation serves as a stressor that triggers inflammation within the central nervous system, a process known as neuroinflammation. This inflammatory response plays a crucial role in the development of depression by upregulating the expression of inflammatory mediators that contribute to symptoms such as anxiety, hopelessness, and loss of pleasure.
METHODS: In this study, sleep deprivation was utilized as a method to induce anxiety and depressive-like behaviors in mice. The behavioral changes in the mice were then evaluated using the EZM, EPM, TST, FST, and SPT. H&E staining and Nissl staining was used to detect morphological changes in the medial prefrontal cortical (mPFC) regions. Elisa to assess serum CORT levels. Detection of mRNA levels and protein expression of clock genes, high mobility genome box-1 (Hmgb1), silent message regulator 6 (Sirt6), and pro-inflammatory factors by RT-qPCR, Western blotting, and immunofluorescence techniques.
RESULTS: Sleep deprivation resulted in decreased exploration of unfamiliar territory, increased time spent in a state of despair, and lower sucrose water intake in mice. Additionally, sleep deprivation led to increased secretion of serum CORT and upregulation of clock genes, IL6, IL1β, TNFα, Cox-2, iNOS, Sirt6, and Hmgb1. Sleep.
CONCLUSIONS: Sleep deprivation induces anxiety-depressive-like behaviors and neuroinflammation in the brain. Transcription of clock genes and activation of the Sirt6/Hmgb1 pathway may contribute to inflammatory responses in the mPFC.