UNASSIGNED:评估五种广泛的商业产品在中国的粘菌素和多粘菌素B敏感性试验中对mcr阳性和阴性大肠杆菌和肺炎克雷伯菌的性能。
UNASSIGNED:共收集了132个大肠杆菌和83个肺炎克雷伯菌菌株(包括68个mcr-1阳性大肠杆菌和28个mcr-8阳性肺炎克雷伯菌)。我们分析了粘菌素敏感性的表现(用Vitek2和PhoenixM50)和多粘菌素B敏感性的表现(用DL-96II,MA120和多粘菌素B敏感性测试条;POLE-条)。肉汤微量稀释用作金标准。分类协议(CA),基本协议(EA),主要错误(ME),并计算了非常大的误差(VME)进行比较。
未经批准:对于大肠杆菌,总CA,EA,我,VME对粘菌素的影响如下:Vitek2,98.5%/98.5%/0%/2.9%;凤凰城M50,98.5%/97.7%/0%/2.9%。总CA,EA,我,和VME对多粘菌素B的影响如下:POLE-条,99.2%/63.6%/1.6%/0%;MA120,70.0%/-/0%/58.8%;和DL-96II,80.2%/-/1.6%/36.8%。只有Vitek2和PhoenixM50对mcr-1阳性大肠杆菌表现令人满意。对于肺炎克雷伯菌,总CA,EA,我,VME对粘菌素的影响如下:Vitek2,73.2%/72.0%/61.6%;凤凰城M50,74.7%/74.7%/0%/58.3%。总CA,EA,我,和VME对多粘菌素B的影响如下:POLE-条,91.6%/74.7%/2.1%/16.7%;MA120、92.8%/-/2.1%/13.9%;DL-96II、92.2%/-/2.1%/8.3%。所有系统对于mcr-8阳性肺炎克雷伯菌均不令人满意。当检测mcr阴性菌株的敏感性时,所有系统都表现出卓越的性能。
UNASSIGNED:Vitek2和PhoenixM50对大肠杆菌的粘菌素显示出可接受的性能,无论mcr-1表达如何,而DL-96II,MA120和POLE试条对mcr-1阳性菌株的表现更差。此外,mcr-8极大地影响了粘菌素和多粘菌素B对肺炎克雷伯菌分离株的所有系统的性能。
UNASSIGNED: To evaluate the performance of five widespread commercial products for colistin and polymyxin B susceptibility testing in
China for mcr-positive and -negative Escherichia coli and Klebsiella pneumoniae.
UNASSIGNED: A total of 132 E. coli and 83 K. pneumoniae strains (including 68 mcr-1-positive E. coli and 28 mcr-8-positive K. pneumoniae) were collected. We analysed the performance of colistin susceptibility (with Vitek 2 and Phoenix M50) and the performance of polymyxin B susceptibility (with DL-96II, MA120, and a Polymyxin B Susceptibility Test strip; POL E-strip). Broth microdilution was used as the gold standard. Categorical agreement (CA), essential agreement (EA), major error (ME), and very major error (VME) were calculated for comparisons.
UNASSIGNED: For E. coli, the total CA, EA, ME, and VME to colistin were as follows: Vitek 2, 98.5%/98.5%/0%/2.9%; and Phoenix M50, 98.5%/97.7%/0%/2.9%. The total CA, EA, ME, and VME to polymyxin B were as follows: POL E-strip, 99.2%/63.6%/1.6%/0%; MA120, 70.0%/-/0%/58.8%; and DL-96II, 80.2%/-/1.6%/36.8%. Only Vitek 2 and Phoenix M50 presented satisfactory performances for mcr-1-positive E. coli. For K. pneumoniae, the total CA, EA, ME, and VME to colistin were as follows: Vitek 2, 73.2%/72.0%/0%/61.6%; and Phoenix M50, 74.7%/74.7%/0%/58.3%. The total CA, EA, ME, and VME to polymyxin B were as follows: POL E-strip, 91.6%/74.7%/2.1%/16.7%; MA120, 92.8%/-/2.1%/13.9%; and DL-96II, 92.2%/-/2.1%/8.3%. All systems were unsatisfactory for mcr-8-positive K. pneumoniae. When the susceptibility of mcr-negative strains was tested, all systems presented excellent performance.
UNASSIGNED: Vitek 2 and Phoenix M50 with colistin for E. coli showed acceptable performance regardless of mcr-1 expression, while DL-96II, MA120, and the POL E-strip performed worse for mcr-1-positive strains. Furthermore, mcr-8 greatly affected the performance of all systems with both colistin and polymyxin B for K. pneumoniae isolates.