N-氯-N-氟苯磺酰胺(CFBSA),是一种新型的氯化试剂,具有潜在的抗菌活性。在这项研究中,CFBSA被确认为针对不同革兰氏阴性菌的广泛抗菌和杀菌药物,革兰氏阳性菌和真菌,同时发现它对真核细胞的细胞毒性较低。此外,微生物形态学分析和氧化应激试验用于确定CFBSA的抗菌机制。根据结果,CFBSA可能在细胞膜上具有靶标,并通过破坏其细胞膜来杀死微生物。然后,CFBSA首先通过静电纺丝与PLCL/SF组合并应用于伤口敷料。通过SEM研究了负载CFBSA的纳米纤维毡的不同PLCL/SF的表征,水接触角,FTIR,细胞相容性和抗菌试验。负载CFBSA的PLCL/SF纳米纤维垫显示出优异的抗微生物活性。为了平衡生物相容性和抗菌效率,选择SP-2.5作为进一步应用负载CFBSA的PLCL/SF的理想负载浓度。总之,静电纺丝CFBSA负载的PLCL/SF纳米纤维垫具有广谱抗菌和杀菌活性以及良好的生物相容性,显示出巨大的伤口敷料潜力。
N-chloro-N-fluorobenzenesulfonylamide (CFBSA), was a novel chlorinating reagent, which exhibits potential antibacterial activities. In this study, CFBSA was confirmed as a wide-broad
antimicrobial and bactericidal drug against different gram-negative bacteria, gram-positive bacteria and fungi, while it was found to have low cytotoxicity for eukaryotic cells. In addition, microorganism morphology assay and oxidative stress test was used to determine the
antimicrobial mechanisms of CFBSA. According to the results, CFBSA probably had a target on cell membrane and killed microorganism by disrupting its cell membrane. Then, CFBSA was first combined with PLCL/SF via electrospinning and applied in wound dressings. The characterization of different PLCL/SF of CFBSA-loaded nanofibrous mats was investigated by SEM, water contact angle, FTIR, cell compatibility and
antimicrobial test. CFBSA-loaded PLCL/SF nanofibrous mats showed excellent
antimicrobial activities. In order to balance of the biocompatibility and antibacterial efficiency, SP-2.5 was selected as the ideal loading concentration for further application of CFBSA-loaded PLCL/SF. In conclusion, the electrospun CFBSA-loaded PLCL/SF nanofibrous mat with its broad-spectrum
antimicrobial and bactericidal activity and good biocompatibility showed enormous potential for wound dressing.