PDF(Pubmed)
Abstract：
UNASSIGNED: Widespread opportunistic pathogens pose a serious threat to global health, particularly in susceptible hospital populations. The escalating crisis of antibiotic resistance highlights the urgent need for novel antibacterial agents and alternative treatment approaches. Traditional Chinese Medicine (TCM) and its compounds have deep roots in the treatment of infectious diseases. It has a variety of active ingredients and multi-target properties, opening up new avenues for the discovery and development of antimicrobial drugs.
UNASSIGNED: This study focuses on assessing the efficacy of the Shensheng-Piwen changed medicinal powder (SPC) extracts against opportunistic pathogen infections by broth microdilution and agar disc diffusion methods. Additionally, biofilm inhibition and eradication assays were performed to evaluate the antibiofilm effects of SPC extracts.
UNASSIGNED: Metabolite profiles were analyzed by LC-MS. Furthermore, the potential synergistic effect between SPC and Metal-Organic Framework (MOF) was investigated by bacterial growth curve analysis. The results indicated that the SPC extracts exhibited antibacterial activity against S. aureus, with a minimum inhibitory concentration (MIC) of 7.8 mg/mL (crude drug concentration). Notably, at 1/2 MIC, the SPC extracts significantly inhibited biofilm formation, with over 80% inhibition, which was critical in tackling chronic and hospital-acquired infections. Metabolomic analysis of S. aureus revealed that SPC extracts induced a notable reduction in the levels of various metabolites, including L-proline, L-asparagine. This suggested that the SPC extracts could interfere with the metabolism of S. aureus. Meanwhile, the growth curve experiment proved that SPC extracts and MOFs had a synergistic antibacterial effect.
UNASSIGNED: In conclusion, the present study highlights the potential of SPC extracts as a novel antibacterial agent against S. aureus infections, with promising biofilm inhibition properties. The observed synergistic effect between SPC extracts and MOFs further supports the exploration of this combination as an alternative treatment approach.
UNASSIGNED: This study focuses on assessing the efficacy of the Shensheng-Piwen changed medicinal powder (SPC) extracts against opportunistic pathogen infections by broth microdilution and agar disc diffusion methods. Additionally, biofilm inhibition and eradication assays were performed to evaluate the antibiofilm effects of SPC extracts.
UNASSIGNED: Metabolite profiles were analyzed by LC-MS. Furthermore, the potential synergistic effect between SPC and Metal-Organic Framework (MOF) was investigated by bacterial growth curve analysis. The results indicated that the SPC extracts exhibited antibacterial activity against S. aureus, with a minimum inhibitory concentration (MIC) of 7.8 mg/mL (crude drug concentration). Notably, at 1/2 MIC, the SPC extracts significantly inhibited biofilm formation, with over 80% inhibition, which was critical in tackling chronic and hospital-acquired infections. Metabolomic analysis of S. aureus revealed that SPC extracts induced a notable reduction in the levels of various metabolites, including L-proline, L-asparagine. This suggested that the SPC extracts could interfere with the metabolism of S. aureus. Meanwhile, the growth curve experiment proved that SPC extracts and MOFs had a synergistic antibacterial effect.
UNASSIGNED: In conclusion, the present study highlights the potential of SPC extracts as a novel antibacterial agent against S. aureus infections, with promising biofilm inhibition properties. The observed synergistic effect between SPC extracts and MOFs further supports the exploration of this combination as an alternative treatment approach.
摘要：
■广泛的机会性病原体对全球健康构成严重威胁，特别是在易感人群中。不断升级的抗生素耐药性危机凸显了对新型抗菌剂和替代治疗方法的迫切需要。中药及其化合物在感染性疾病的治疗中有着深厚的根基。它具有多种活性成分和多目标特性，为发现和开发抗菌药物开辟了新的途径。
■这项研究的重点是通过肉汤微量稀释和琼脂圆盘扩散方法评估神生-皮文变味药粉（SPC）提取物对机会性病原体感染的功效。此外,进行生物膜抑制和根除试验以评估SPC提取物的抗生物膜作用。
■用LC-MS分析代谢物分布。此外，通过细菌生长曲线分析研究了SPC和金属有机框架（MOF）之间的潜在协同作用。结果表明，SPC提取物对金黄色葡萄球菌具有抗菌活性，最低抑制浓度（MIC）为7.8mg/mL（原料药浓度）。值得注意的是,在1/2MIC，SPC提取物显著抑制生物膜形成，抑制超过80%,这对于解决慢性和医院获得性感染至关重要。金黄色葡萄球菌的代谢组学分析显示，SPC提取物诱导各种代谢物水平显着降低，包括L-脯氨酸,L-天冬酰胺.这表明SPC提取物可能干扰金黄色葡萄球菌的代谢。同时,生长曲线实验证明SPC提取物与MOFs具有协同抗菌作用。
■总而言之，本研究强调了SPC提取物作为抗金黄色葡萄球菌感染的新型抗菌剂的潜力，具有良好的生物膜抑制性能。在SPC提取物和MOFs之间观察到的协同作用进一步支持了该组合作为替代治疗方法的探索。
■这项研究的重点是通过肉汤微量稀释和琼脂圆盘扩散方法评估神生-皮文变味药粉（SPC）提取物对机会性病原体感染的功效。此外,进行生物膜抑制和根除试验以评估SPC提取物的抗生物膜作用。
■用LC-MS分析代谢物分布。此外，通过细菌生长曲线分析研究了SPC和金属有机框架（MOF）之间的潜在协同作用。结果表明，SPC提取物对金黄色葡萄球菌具有抗菌活性，最低抑制浓度（MIC）为7.8mg/mL（原料药浓度）。值得注意的是,在1/2MIC，SPC提取物显著抑制生物膜形成，抑制超过80%,这对于解决慢性和医院获得性感染至关重要。金黄色葡萄球菌的代谢组学分析显示，SPC提取物诱导各种代谢物水平显着降低，包括L-脯氨酸,L-天冬酰胺.这表明SPC提取物可能干扰金黄色葡萄球菌的代谢。同时,生长曲线实验证明SPC提取物与MOFs具有协同抗菌作用。
■总而言之，本研究强调了SPC提取物作为抗金黄色葡萄球菌感染的新型抗菌剂的潜力，具有良好的生物膜抑制性能。在SPC提取物和MOFs之间观察到的协同作用进一步支持了该组合作为替代治疗方法的探索。
