周围神经吻合后,吻合部位容易与周围组织粘连,从而影响神经修复的有效性。这项研究探讨了去细胞外膜作为外周神经修复中抗粘附生物膜的发展和功效。首先,整个神经外膜是从新鲜的猪坐骨神经中提取的,随后是去细胞化过程。然后彻底评估去细胞化效率。随后,对脱细胞神经外膜进行蛋白质组学分析,以确定剩余的生物活性成分.为了确保生物安全,脱细胞神经外膜进行了细胞毒性试验,溶血试验,细胞亲和力测定,和评估皮下植入后的免疫反应。最后,使用大鼠坐骨神经横断和吻合模型确定生物膜的功能。结果表明,脱细胞过程有效地从神经外膜去除细胞成分,同时保留了许多生物活性分子,这种脱细胞神经外膜能有效防止粘连,同时促进神经修复和功能恢复。总之,脱细胞神经外膜代表了一种新颖且有前景的抗粘连生物膜,可增强周围神经修复的手术效果。
Following peripheral nerve anastomosis, the anastomotic site is prone to adhesions with surrounding tissues, consequently impacting the effectiveness of nerve repair. This study explores the development and efficacy of a decellularized epineurium as an anti-adhesive biofilm in peripheral nerve repair. Firstly, the entire epineurium was extracted from fresh porcine sciatic nerves, followed by a decellularization process. The decellularization efficiency was then thoroughly assessed. Subsequently, the decellularized epineurium underwent proteomic analysis to determine the remaining bioactive components. To ensure biosafety, the decellularized epineurium underwent cytotoxicity assays, hemolysis tests, cell affinity assays, and assessments of the immune response following subcutaneous implantation. Finally, the functionality of the biofilm was determined using a sciatic nerve transection and anastomosis model in rats. The result indicated that the decellularization process effectively removed cellular components from the epineurium while preserving a number of bioactive molecules, and this decellularized epineurium was effective in preventing adhesion while promoting nerve repairment and functional recovery. In conclusion, the decellularized epineurium represents a novel and promising anti-adhesion biofilm for enhancing surgical outcomes of peripheral nerve repair.