PDF(Pubmed)
Abstract：
Polypropylene (PP) mesh is commonly used in abdominal wall repair due to its ability to reduce the risk of organ damage, infections and other complications. However, the PP mesh often leads to adhesion formation and does not promote functional tissue repair. In this study, we synthesized one kind of aldehyde Bletilla striata polysaccharide (BSPA) modified chitosan (CS) hydrogel based on Schiff base reaction. The hydrogel exhibited a porous network structure, a highly hydrophilic surface and good biocompatibility. We wrapped the PP mesh inside the hydrogel and evaluated the performance of the resulting composites in a bilateral 1 × 1.5 cm abdominal wall defect model in rats. The results of gross observation, histological staining and immunohistochemical staining demonstrated the positive impact of the CS hydrogel on anti-adhesion and wound healing effects. Notably, the addition of BSPA to the CS hydrogel further improved the performance of the composites in vivo, promoting wound healing by enhancing collagen deposition and capillary rearrangement. This study suggested that the BSPA-modified CS hydrogel significantly promoted the anti-adhesion, anti-inflammatory and pro-angiogenesis properties of PP meshes during the healing process. Overall, this work offers a novel approach to the design of abdominal wall repair patches.
摘要：
聚丙烯（PP）网因其能够降低器官损伤的风险而常用于腹壁修复，感染和其他并发症。然而,PP网片通常导致粘连形成并且不促进功能性组织修复。在这项研究中,基于席夫碱反应合成了一种乙醛白及多糖(BSPA)改性壳聚糖(CS)水凝胶。水凝胶表现出多孔网络结构，一个高度亲水的表面和良好的生物相容性。我们将PP网包裹在水凝胶内，并在大鼠双侧1×1.5cm腹壁缺损模型中评估所得复合材料的性能。粗略观察的结果，组织学染色和免疫组织化学染色表明CS水凝胶对抗粘连和伤口愈合效果的积极影响。值得注意的是,在CS水凝胶中加入BSPA进一步提高了复合材料的体内性能,通过增强胶原沉积和毛细血管重排促进伤口愈合。这项研究表明，BSPA修饰的CS水凝胶显着促进了抗粘连，PP网在愈合过程中的抗炎和促血管生成特性。总的来说,这项工作为设计腹壁修复补片提供了一种新颖的方法。
