未经证实:病原体感染会影响葡萄浆果的收获后保质期。在一项初步研究中,velezensis芽孢杆菌产生的代谢物显着抑制了葡萄采后病原菌奥氏青霉的生长。
UNASSIGNED:为了研究维氏芽孢杆菌与奥氏疟原虫之间的相互作用机制,使用IlluminaNovaSeq平台为P.olsoniiWHG5生成了基因组草图,并分析了WHG5的转录组变化,以响应暴露于维氏芽孢杆菌代谢物(10%v/v)。
未经证实:与孢子形成相关的基因的表达水平,包括GCY1,brla,和abaA,与对照组相比下调。此外,观察到分生孢子的孢子变形和异常膨胀。关键酶的表达,包括果糖2,6-二磷酸和甘露醇-2-脱氢酶,被下调了,表明WHG5的糖酵解途径受到维氏芽孢杆菌代谢产物的不利影响。KEGG途径富集分析表明,谷胱甘肽代谢和抗氧化酶系统参与了对维氏芽孢杆菌代谢产物的反应。发病相关基因的下调,PG1和POT1表明维氏芽孢杆菌代谢产物降低了奥氏芽孢杆菌的致病性。B.velezensis代谢物通过影响葡萄糖代谢来破坏奥氏疟原虫中活性氧的稳态,导致孢子变形和生长中断。此外,关键发病相关基因的表达下调,从而降低奥氏疟原虫的致病性。
UNASSIGNED:这项研究提供了有关奥氏疟原虫对维氏芽孢杆菌代谢产物的反应的见解,并确定了可能在抑制葡萄收获后腐败的生物控制策略中有用的潜在靶基因。
UNASSIGNED: Pathogen infection influences the post-harvest shelf life of grape berries. In a preliminary study, metabolites produced by Bacillus velezensis significantly inhibited the growth of the grape postharvest pathogen Penicillium olsonii.
UNASSIGNED: To investigate the mechanism of interaction between B. velezensis and P. olsonii, a draft genome was generated for P. olsonii WHG5 using the Illumina NovaSeq platform, and the transcriptomic changes in WHG5 were analyzed in response to the exposure to B. velezensis metabolites (10% v/v).
UNASSIGNED: The expression levels of genes associated with sporulation, including GCY1, brlA, and abaA, were down-regulated compared with those of the control. In addition, spore deformation and abnormal swelling of the conidiophore were observed. The expression of crucial enzymes, including fructose 2,6-bisphosphate and mannitol-2-dehydrogenase, was down-regulated, indicating that the glycolytic pathway of WHG5 was adversely affected by B. velezensis metabolites. The KEGG pathway enrichment analysis revealed that glutathione metabolism and the antioxidant enzyme system were involved in the response to B. velezensis metabolites. The down-regulation of the pathogenesis-related genes, PG1 and POT1, suggested that B. velezensis metabolites decreased the pathogenicity of P. olsonii. B. velezensis metabolites disrupted the homeostasis of reactive oxygen species in P. olsonii by affecting glucose metabolism, resulting in spore deformation and disruption of growth. In addition, the expression of key pathogenesis-related genes was down-regulated, thereby reducing the pathogenicity of P. olsonii.
UNASSIGNED: This study provides insights into the responses of P. olsonii to B. velezensis metabolites and identifies potential target genes that may be useful in biocontrol strategies for the suppression of post-harvest spoilage in grapes.