■N6-甲基腺苷(m6A)是最普遍的mRNA甲基化,在各种生理过程中起着至关重要的作用,包括色素沉着。然而,监管机制,包括长链非编码RNA(lncRNAs)m6A甲基化导致绵羊皮肤色素沉着仍不清楚。这项研究的目的是鉴定潜在的lncRNAs和与色素沉着相关的lncRNAs的m6A甲基化。
■进行RNA-seq和MeRIP-seq以研究lncRNAs的表达和lncRNAs在黑色和白色绵羊皮肤中的m6A甲基化。此外,定量实时聚合酶链反应(qRT-PCR)用于验证与RNA-seq和MeRIP-seq数据的一致性。
■在两种绵羊皮肤颜色之间检测到168种差异表达的lncRNA。富含ECM-受体相互作用途径的差异表达lncRNAs,Rap1信号通路,非同源末端连接可能在色素沉着中起重要作用。我们在黑白羊皮中发现了577个m6A峰和617个m6A峰,分别,其中20个m6A峰表现出显著差异。羊皮中的富集图案为“GGACU”,与共识基序“RRACH”(R=A或G,H=A,C或U)。富含PI3K-Akt信号通路和Wnt信号通路的不同甲基化lncRNAs可能参与皮肤色素沉着。ENSOARG00020015168是黑羊胫骨中具有高表达和甲基化(Hyper-Up)的独特lncRNA。构建了一个lncRNA-mRNA网络,与色素沉着相关的基因,如PSEN2、CCND3、COL2A1和ERCC3。
■对黑白羊皮肤中lncRNAs的m6A修饰进行了综合分析,为调节色素沉着提供新的候选人。
UNASSIGNED: N6-methyladenosine (m6A) is the most prevalent methylation of mRNA and plays crucial roles in various physiological processes, including pigmentation. Yet, the regulatory mechanisms, including long noncoding RNAs (lncRNAs) m6A methylation contributing to pigmentation in sheep skin remains unclear. The purpose of this study was to identify potential lncRNAs and the m6A methylation of lncRNAs associated with pigmentation.
UNASSIGNED: RNA-seq and MeRIP-seq were performed to study the expression of lncRNAs and the m6A methylation of lncRNAs in black and white sheep skin. Furthermore, quantitative real-time polymerase chain reaction (qRT‒PCR) was used to verify the consistency with the RNA-seq and MeRIP-seq data.
UNASSIGNED: 168 differentially expressed lncRNAs were detected between the two sheep skin colors. The differentially expressed lncRNAs enriched in the pathway of ECM-receptor interaction, Rap1 signaling pathway, and Non-homologous end-joining may play essential roles in pigmentation. We identified 577 m6A peaks and 617 m6A peaks in black and white sheep skin, respectively, among which 20 m6A peaks showed significant differences. The enriched motif in sheep skin was \"GGACU\", which aligned with the consensus motif \"RRACH\" (R=A or G, H=A, C or U). Differently methylated lncRNAs enriched in PI3K-Akt signaling pathway and Wnt signaling pathway might participate in skin pigmentation. ENSOARG00020015168 was the unique lncRNA with high expression and methylation (Hyper-Up) in black sheep shin. A lncRNA-mRNA network was constructed, with pigmentation-related genes, such as PSEN2, CCND3, COL2A1, and ERCC3.
UNASSIGNED: The m6A modifications of lncRNAs in black and white colored sheep skin were analyzed comprehensively, providing new candidates for the regulation of pigmentation.