黄连木(P.chinensis),雌雄异株的植物,在中国已被广泛发现。在农业生产中,雌性中国植物比雄性植物更重要。因为它们的种子可以作为生物柴油的理想原料。然而,由于可用的转录组学和基因组信息的缺乏,在幼苗阶段很难区分中国山竹植物的性别。在这项工作中,进行Illumina配对末端RNA测序测定,以解开雌性和雄性中国金牛花蕾的转录组学谱。总的来说,从雌性和雄性cDNA文库获得50,925,088和51,470,578个干净的读数,分别。经过质量检查和重新组装,总共筛选了83,370个基因,平均长度为1.3kb。总的来说,在至少一个NR中可以匹配64,539个单基因(77.48%),NT,Swiss-Prot,COG,KEGG,和GO数据库,其中71个与中国紫菜花发育有关。此外,在17,028个单基因中鉴定出21,662个简单序列重复(SSR)基序,单核苷酸基序是中华黄曲霉中最主要的重复类型(52.59%),其次是二核苷酸(22.29%),三核苷酸(20.15%)。最丰富的重复序列是AG/CT(13.97%),其次是AAC/GTT(6.75%)和AT/TA(6.10%)。基于这些SSR,设计了983个EST-SSR引物,其中151个是随机选择进行验证的。在这些经过验证的EST-SSR标记中,发现25个SSR标记在雄性和雌性植物之间具有多态性。一个SSR标记,namelyPCSSR55,在雌性植物中表现出优异的特异性,可以清楚地区分雄性和雌性。总之,我们的研究结果不仅表明EST-SSR标记在区分雄性和雌性中国紫菜方面非常有效,而且为植物幼苗的性别确定提供了坚实的框架。
Pistacia chinensis Bunge (P. chinensis), a dioecious plant species, has been widely found in
China. The female P. chinensis plants are more important than male plants in agricultural production, as their seeds can serve as an ideal feedstock for biodiesel. However, the sex of P. chinensis plants is hard to distinguish during the seedling stage due to the scarcity of available transcriptomic and genomic information. In this work, Illumina paired-end RNA sequencing assay was conducted to unravel the transcriptomic profiles of female and male P. chinensis flower buds. In total, 50,925,088 and 51,470,578 clean reads were obtained from the female and male cDNA libraries, respectively. After quality checks and de novo assembly, a total of 83,370 unigenes with a mean length of 1.3 kb were screened. Overall, 64,539 unigenes (77.48%) could be matched in at least one of the NR, NT, Swiss-Prot, COG, KEGG, and GO databases, 71 of which were putatively related to the floral development of P. chinensis. Additionally, 21,662 simple sequence repeat (SSR) motifs were identified in 17,028 unigenes of P. chinensis, and the mononucleotide motif was the most dominant type of repeats (52.59%) in P. chinensis, followed by dinucleotide (22.29%), trinucleotide (20.15%). The most abundant repeats were AG/CT (13.97%), followed by AAC/GTT (6.75%) and AT/TA (6.10%). Based on these SSR, 983 EST-SSR primers were designed, 151 of which were randomly chosen for validation. Of these validated EST-SSR markers, 25 SSR markers were found to be polymorphic between male and female plants. One SSR marker, namelyPCSSR55, displayed excellent specificity in female plants, which could clearly distinguish between male and female P. chinensis. Altogether, our findings not only reveal that the EST-SSR marker is extremely effective in distinguishing between male and female P. chinensis but also provide a solid framework for sex determination of plant seedlings.