PDF(Pubmed)
Abstract：
Accurate gender identification is crucial for the study of bird reproduction and evolution. The current study aimed to explore and evaluate the effectiveness of a noninvasive method for gender identification in Yangzhou geese. In this experiment, 600 goose eggs were collected. Hair root tissues were used for PCR amplification, molecular sequencing, and anal inversion for early sex recognition in goslings. According to the DNA amplification results for the feather pulp tissue of 2-wk-old geese, bands appeared at 436 bp (CHD1-Z) and 330 bp (CHD1-W) upon gel electrophoresis. This method considered the base of goose feathers to accelerate the process of gender recognition. By examining the sex of anatomized poultry for verification, the accuracy rate of PCR gel electrophoresis and molecular sequencing sex identification was 100%, whereas the average accuracy rate of anal inversion was 97.41%. In the comparison of feather growth trends at 0 to 18 wk of age, the feather root weight (FRW), feather root length (FRL), feather branch length (FBL), and feather shaft diameter (FSD) of Yangzhou goose of the same age were not significantly different between males and females (P > 0.05). At 6 wk of age, the FRW, FRL, and FSD in males and FRL in females increased rapidly; their growth increased by 84.43, 67.58, 45.10, and 69.42%, respectively. At 10 wk of age, the male FRL, male FBL, and female FBL increased by 37.31, 34.81, and 21.72, respectively. The Boltzmann model was found to be the best-fitting model for the feathers of male Yangzhou geese. Early sex identification based on feather growth trends between the sexes is not feasible. This study provides a convenient and reliable technical means for early sex identification of waterfowl and serves as an ecological strategy for protecting the reproduction of poultry populations.
摘要：
准确的性别识别对于研究鸟类的繁殖和进化至关重要。本研究旨在探索和评价一种无创性别识别方法在扬州鹅中的应用效果。在这个实验中,收集600个鹅卵。毛根组织用于PCR扩增，分子测序，和肛门倒置以早期识别小鹅的性别。根据2周龄鹅羽肉组织的DNA扩增结果，凝胶电泳在436bp（CHD1-Z）和330bp（CHD1-W）处出现条带。这种方法考虑了鹅羽毛的基础，以加速性别识别的过程。通过检查解剖家禽的性别进行验证，PCR凝胶电泳和分子测序性别鉴定的准确率为100%,而肛门内翻的平均准确率为97.41%。在0至18周龄的羽毛生长趋势比较中，羽毛根重量（FRW），羽毛根长度（FRL），羽毛分支长度（FBL），同龄扬州鹅的羽毛轴直径（FSD）在雄性和雌性之间没有显着差异（P>0.05）。6周时，FRW,FRL,男性FSD和女性FRL增长迅速；它们的增长分别增长了84.43、67.58、45.10和69.42%，分别。10岁时，男性FRL,男性FBL,女性FBL分别增加37.31、34.81和21.72。Boltzmann模型被认为是扬州鹅羽毛的最佳拟合模型。根据性别之间的羽毛生长趋势进行早期性别识别是不可行的。本研究为水禽的早期性别鉴定提供了方便可靠的技术手段，并作为保护家禽种群繁殖的生态策略。
