Abstract：
1. Sex chromosomes of emus are largely homomorphic. Therefore, the standard methodology for molecular sexing based on screening intron length variations in sex-linked genes is not applicable. However, emu sexing requires costly and time-consuming PCR-RFLP or multiplex PCR methods.2. This experiment used a directed PCR amplification and capillary electrophoresis sexing protocol. Two distinct peaks were observed in females (ZW), while only one peak was observed in males (ZZ).3. This sexing technique proved to be rapid, non-invasive, and highly sensitive and may be useful for verifying the sex ratio and breeding management of emus.
摘要：
1.emus的性染色体在很大程度上是同态的。因此,分子性别鉴定的标准方法是基于性别连锁基因中内含子长度变异的筛选方法。然而,emu性别鉴定需要昂贵且耗时的PCR-RFLP或多重PCR方法。该实验使用定向PCR扩增和毛细管电泳性别鉴定方案。在女性（ZW）中观察到两个不同的峰，而在男性（ZZ）中只观察到一个峰。这种性别鉴定技术被证明是快速的，非侵入性，并且高度敏感，可能有助于验证e的性别比例和繁殖管理。
