■江华苦茶(JHKC)是一种特殊的茶种质,具有丰富的特殊次生代谢产物,包括theacrine,非差向异构黄烷醇和甲基化黄烷醇。此外,初级代谢物为次级代谢物的生产提供前体和能量。然而,JHKC不同组织中初级和次级代谢产物的积累模式尚不清楚。
■不同JHKC组织中初级和次级代谢产物及相关代谢途径(初级和次级代谢)的变化(芽,第1-4叶,和新茎)通过超高效液相色谱四极杆飞行时间质谱(UHPLC-QTOF/MS)的代谢组学分析进行了研究。
■在主要与淀粉和蔗糖途径有关的68种初级和51种次级代谢产物中观察到显着差异,氨基酸,咖啡因,黄烷醇代谢和TCA循环。芽表现出更高水平的葡萄糖-6-磷酸,柠檬酸,大多数氨基酸,可可碱,儿茶素没食子酸酯,表儿茶素没食子酸酯,原花青素,和theasinensins;第一片叶子显示出更高水平的咖啡因和表没食子儿茶素-3-没食子酸酯;第四片叶子含有更高水平的大多数单糖,theacrine,和表没食子儿茶素-3-O-(3’-O-甲基)-没食子酸酯。此外,初级代谢产物与重要的次级代谢产物具有一定的相关性。
■这项研究提供了对JHKC初级和次级代谢物的全面了解,并为将来有效利用JHKC的专门代谢物提供了指南。
UNASSIGNED: Jianghua Kucha (JHKC) is a special tea germplasm with enriched specialized secondary metabolites, including theacrine, non-epimeric flavanols and methylated flavanols. Moreover, primary metabolites provide precursors and energy for the production of secondary metabolites. However, the accumulation patterns of primary and secondary metabolites in different tissues of JHKC are unclear.
UNASSIGNED: The changes of primary and secondary metabolites and related metabolic pathways (primary and secondary metabolism) in different JHKC tissues (the bud, 1st-4th leaves, and new stem) were investigated via metabolomics analysis with ultra-high-performance liquid chromatography quadrupole time-of-flight mass spectrometry (UHPLC-QTOF/MS).
UNASSIGNED: Significant differences were observed in 68 primary and 51 secondary metabolites mainly related with the pathways of starch and sucrose, amino acids, caffeine, and flavanols metabolism and TCA cycle. The bud exhibited higher levels of glucose-6-phosphate, citric acid, most amino acids, theobromine, catechin-gallate, epicatechin-gallate, procyanidins, and theasinensins; the 1st leaf showed higher levels of caffeine and epigallocatechin-3-gallate; and the 4th leaf contained higher levels of most monosaccharides, theacrine, and epigallocatechin-3-O-(3\"-O-methyl)-gallate. In addition, primary metabolites and important secondary metabolites had certain correlations.
UNASSIGNED: This study provides comprehensive insight into primary and secondary metabolites in JHKC and offers guidelines for efficiently utilizing specialized metabolites of JHKC in the future.