Exploring Antioxidant and α-Glucosidase Inhibitory Activities in Mulberry Leaves (Morus alba L.) across Growth Stages: A Comprehensive Metabolomic Analysis with Chemometrics.

探索跨生长阶段桑叶 （ Morus alba L. ） 的抗氧化和 α - 葡萄糖苷酶抑制活性 ： 化学计量学的综合代谢组学分析。

• 影响指数: 4.927
• 发表时间: 2023 Dec 27
• 来源期刊: Molecules
• DOI: 10.3390/molecules29010171
• PMID: 38202754
• 文章类型: Journal Article
• 中科院分区: Q3
• 作者列表: Li W,Hao S,Li H,An Q,Yang L,Guo B,Xue Z,Liu Y,Guo L,Zheng Y,Zhang D

关键词: Morus alba L.; antioxidant activity; different growth stages; primary metabolites; secondary metabolites

Mesh: Morus; Antioxidants / pharmacology; Chemometrics; alpha-Glucosidases

Abstract

Metabolic product accumulation exhibited variations among mulberry (Morus alba L.) leaves (MLs) at distinct growth stages, and this assessment was conducted using a combination of analytical techniques including high-performance liquid chromatography (HPLC), gas chromatography-mass spectrometry (GC-MS), and liquid chromatography-mass spectrometry (LC-MS). Multivariate analysis was applied to the data, and the findings were correlated with antioxidant activity and α-glucosidase inhibitory effects in vitro. Statistical analyses divided the 27 batches of MLs at different growth stages into three distinct groups. In vitro assays for antioxidant activity and α-glucosidase inhibition revealed that IC50 values were highest at the Y23 stage, which corresponds to the \'Frost Descends\' solar term. In summary, the results of this study indicate that MLs at different growth stages throughout the year can be categorized into three primary growth stages using traditional Chinese solar terms as reference points, based on the observed variations in metabolite content.

摘要

代谢产物的积累在不同的生长阶段在桑树（MorusalbaL.）叶（MLs）之间表现出变化，这项评估是使用包括高效液相色谱（HPLC）在内的分析技术的组合进行的，气相色谱-质谱(GC-MS),和液相色谱-质谱(LC-MS)。对数据进行多变量分析，研究结果与体外抗氧化活性和α-葡萄糖苷酶抑制作用有关。统计分析将处于不同生长阶段的27批MLs分成三个不同的组。体外抗氧化活性和α-葡萄糖苷酶抑制作用的测定显示，IC50值在Y23阶段最高，这对应于“霜降”节气。总之,这项研究的结果表明，使用中国传统节气作为参考点，可以将全年不同生长阶段的MLs分为三个主要生长阶段，根据观察到的代谢物含量变化。