PDF(Pubmed)
Abstract：
BACKGROUND: Weed control is essential for agricultural floor management in vineyards and the inter-row mulching is an eco-friendly practice to inhibit weed growth via filtering out photosynthetically active radiation. Besides weed suppression, inter-row mulching can influence grapevine growth and the accumulation of metabolites in grape berries. However, the complex interaction of multiple factors in the field challenges the understanding of molecular mechanisms on the regulated metabolites. In the current study, black geotextile inter-row mulch (M) was applied for two vintages (2016-2017) from anthesis to harvest. Metabolomics and transcriptomics analysis were conducted in two vintages, aiming to provide insights into metabolic and molecular responses of Cabernet Sauvignon grapes to M in a semi-arid climate.
RESULTS: Upregulation of genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered heat stress, resulting in lower sugar-acid ratio at harvest. Key genes responsible for enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes, and norisoprenoids in M grapes were identified. In addition, several modules significantly correlated with the metabolic biomarkers through weighted correlation network analysis, and the potential key transcription factors regulating the above metabolites including VviGATA11, VviHSFA6B, and VviWRKY03 were also identified.
CONCLUSIONS: This study provides a valuable overview of metabolic and transcriptomic responses of M grapes in semi-arid climates, which could facilitate understanding the complex regulatory network of metabolites in response to microclimate changes.
RESULTS: Upregulation of genes related to photosynthesis and heat shock proteins confirmed that M weakened the total light exposure and grapes suffered heat stress, resulting in lower sugar-acid ratio at harvest. Key genes responsible for enhancements in phenylalanine, glutamine, ornithine, arginine, and C6 alcohol concentrations, and the downward trend in ε-viniferin, anthocyanins, flavonols, terpenes, and norisoprenoids in M grapes were identified. In addition, several modules significantly correlated with the metabolic biomarkers through weighted correlation network analysis, and the potential key transcription factors regulating the above metabolites including VviGATA11, VviHSFA6B, and VviWRKY03 were also identified.
CONCLUSIONS: This study provides a valuable overview of metabolic and transcriptomic responses of M grapes in semi-arid climates, which could facilitate understanding the complex regulatory network of metabolites in response to microclimate changes.
摘要：
背景：杂草控制对于葡萄园的农业地面管理至关重要，行间覆盖是一种生态友好的做法，可以通过过滤掉光合有效辐射来抑制杂草生长。除了抑制杂草,行间覆盖会影响葡萄的生长和葡萄浆果中代谢产物的积累。然而,该领域多种因素的复杂相互作用挑战了对调节代谢物分子机制的理解。在目前的研究中,黑色土工布行间覆盖物（M）应用于从花期到收获的两个年份（2016-2017年）。在两个年份进行了代谢组学和转录组学分析，旨在提供有关赤霞珠葡萄在半干旱气候下对M的代谢和分子响应的见解。
结果：与光合作用和热休克蛋白相关的基因上调证实，M减弱了总光照，葡萄遭受了热胁迫，导致收获时糖酸比降低。负责增强苯丙氨酸的关键基因，谷氨酰胺,鸟氨酸,精氨酸和C6酒精浓度，和ε-葡萄藤素的下降趋势，花青素,黄酮醇，萜烯,并鉴定了M葡萄中的降异戊二烯类。此外,通过加权相关网络分析，几个模块与代谢生物标志物显着相关，以及调节上述代谢物的潜在关键转录因子，包括VviGATA11，VviHSFA6B，和VviWRKY03也被鉴定。
结论：这项研究提供了半干旱气候下M葡萄的代谢和转录响应的有价值的概述，这可以促进理解代谢产物响应小气候变化的复杂调节网络。
结果：与光合作用和热休克蛋白相关的基因上调证实，M减弱了总光照，葡萄遭受了热胁迫，导致收获时糖酸比降低。负责增强苯丙氨酸的关键基因，谷氨酰胺,鸟氨酸,精氨酸和C6酒精浓度，和ε-葡萄藤素的下降趋势，花青素,黄酮醇，萜烯,并鉴定了M葡萄中的降异戊二烯类。此外,通过加权相关网络分析，几个模块与代谢生物标志物显着相关，以及调节上述代谢物的潜在关键转录因子，包括VviGATA11，VviHSFA6B，和VviWRKY03也被鉴定。
结论：这项研究提供了半干旱气候下M葡萄的代谢和转录响应的有价值的概述，这可以促进理解代谢产物响应小气候变化的复杂调节网络。
