Tebufenpyrad广泛用于控制果园中的叶螨,并可能通过径流进入淡水系统,喷雾漂移,等等。很少有论文报道农药对虾等水生类群种群动态的副作用,腹足类,大型植物,浮游植物,和细菌。这里,我们测试了单一应用特布芬比对新卡西汀的影响,Physafontinalis,苦瓜,Simocephalusvetulus,中国多伦霉素,等等,通过室内系统。基于TWA(时间加权平均)的最高无观察效应浓度(NOEC)和最低观察效应浓度(LOEC),按湿重计算,分别为0.67和2.33μg/L,分别,剂量相关效应持续21d。根据我们的研究,尽管受到P.fontinalis的干扰,壳聚糖酶仍可用于量化农药对虾的影响,最终通过使用抗体进行校正。因此,NOEC和LOEC被确定为1.41和≤5.64μg/L,分别,高于湿重计算的值。主成分分析(PCA)和主响应曲线(PRC)调查表明,浮游植物,而Physafontinalis,S.vetulus,和D.sinensis受到杀虫剂的刺激。IlluminaMiSeq用于确定系统内细菌群落的变化。PRC和PCA分析结果表明,特布芬比拉德诱导的硝酸盐还原菌群,硝酸盐反硝化,硫代硫酸盐氧化,尿素溶解,和甲醇氧化,同时抑制了纤维素分解的菌群。发现Tebufenpyrad对pH等水质指标有负面影响,DO,NO3-,NO2-,和SO42-,对PO43-,NH4+,和EC。这表明特布芬比拉导致水质恶化。
Tebufenpyrad are widely used for control leaf mites in orchard and may enter freshwater systems through runoff, spray drift, and so on. Few papers have reported the side effect of the pesticide on population dynamics of aquatic taxa such as shrimps, gastropods, macrophytes, phytoplankton, and bacteria. Here, we tested the effect of a single application of tebufenpyrad on Neocaridina palmata, Physa fontinalis, Ceratophyllum demersum, Simocephalus vetulus, Dolerocypris sinensis, and so on, by indoor systems. The TWA (Time-weighted average)-based highest no observed effect concentration (NOEC) and lowest observed effect concentration (LOEC) for Neocaridina palmata, which were counted by the wet weight, were 0.67 and 2.33 μg/L, respectively, and the dose-related effect lasted 21 d. According to our study, chitobiase could be used to quantify the effects of the pesticide on shrimp despite the interference from P. fontinalis, which was finally corrected by employing of antibodies. The NOEC and LOEC were thus determined to be 1.41 and ≤ 5.64 μg/L, respectively, which were higher than the values that was counted by the wet weight. Principal component analysis (PCA) and principal response curve (PRC) investigation showed that the pesticide suppressed population of C. demersum, and phytoplankton, while the Physa fontinalis, S. vetulus, and D. sinensis were stimulated by the pesticide. Illumina MiSeq was used to determine the alteration in bacterial community within the systems. The results of PRC and PCA analyses showed that tebufenpyrad induced flora of nitrate reducing, nitrate denitrifying, thiosulfate oxidation, ureolysis, and methanol oxidation, while it suppressed flora of cellulolysis. Tebufenpyrad was found to have a negative effect on water quality indicators such as pH, DO, NO3-, NO2-, and SO42-, and a positive effect on PO43-, NH4+, and EC. This suggested that the tebufenpyrad led to water quality deterioration.