PDF(Pubmed)
Abstract：
UNASSIGNED: Pseudomonas aeruginosa (P. aeruginosa) can cause serious infections in many parts of the body and is also an underestimated foodborne pathogen. Intense pulsed light sterilization is recognized for its high sterilization efficiency, flexible and safe operation and ease of installation on production lines, which makes up for the shortcomings of several other physical sterilization technologies.
UNASSIGNED: This experiment studied the killing efficiency of different capacitances (650 μF, 470 μF, and 220 μF) of intense pulsed light on foodborne pathogenic microorganisms P. aeruginosa in the models of liquid food models, 96-well cell plates, and polycarbonate membrane models at room temperature (25°C) and refrigerated (4°C) environments to provide data to support the application of IPL sterilization devices in food processing.
UNASSIGNED: The IPL was very effective in killing P. aeruginosa in the planktonic state as well as in the early and mature biofilm states, meeting target kill rates of 100%, 99.99%, and 94.33% for a given number of exposures. The biofilms formed in the polycarbonate membrane model and the 96-well plate model were more resistant to killing compared to the planktonic state. To achieve the same bactericidal effect, the number of flashes increased with decreasing capacitance.
UNASSIGNED: The bactericidal effect of IPL on P. aeruginosa was significantly influenced by the state of the bacterium. The larger the capacitance the higher the number of pulses and the better the sterilization effect on P. aeruginosa.
UNASSIGNED: This experiment studied the killing efficiency of different capacitances (650 μF, 470 μF, and 220 μF) of intense pulsed light on foodborne pathogenic microorganisms P. aeruginosa in the models of liquid food models, 96-well cell plates, and polycarbonate membrane models at room temperature (25°C) and refrigerated (4°C) environments to provide data to support the application of IPL sterilization devices in food processing.
UNASSIGNED: The IPL was very effective in killing P. aeruginosa in the planktonic state as well as in the early and mature biofilm states, meeting target kill rates of 100%, 99.99%, and 94.33% for a given number of exposures. The biofilms formed in the polycarbonate membrane model and the 96-well plate model were more resistant to killing compared to the planktonic state. To achieve the same bactericidal effect, the number of flashes increased with decreasing capacitance.
UNASSIGNED: The bactericidal effect of IPL on P. aeruginosa was significantly influenced by the state of the bacterium. The larger the capacitance the higher the number of pulses and the better the sterilization effect on P. aeruginosa.
摘要：
■铜绿假单胞菌（P.铜绿假单胞菌）可在身体的许多部位引起严重感染，也是一种被低估的食源性病原体。强脉冲光灭菌因其高灭菌效率而被公认,灵活安全的操作，易于在生产线上安装，弥补了其他几种物理灭菌技术的不足。
■本实验研究了不同电容（650μF，470μF,和220μF）的强脉冲光对液体食品模型模型中的食源性病原微生物铜绿假单胞菌，96孔细胞板，和聚碳酸酯膜模型在室温（25°C）和冷藏（4°C）环境下提供数据，以支持IPL灭菌装置在食品加工中的应用。
■IPL在浮游状态以及早期和成熟生物膜状态下对杀死铜绿假单胞菌非常有效，达到100%的目标杀伤率，99.99%,和94.33%对于给定数量的曝光。与浮游状态相比，在聚碳酸酯膜模型和96孔板模型中形成的生物膜对杀伤的抵抗力更强。达到同样的杀菌效果，闪烁次数随着电容的减小而增加。
■IPL对铜绿假单胞菌的杀菌效果受细菌状态的显着影响。电容越大,脉冲数越多,对铜绿假单胞菌的灭菌效果越好。
■本实验研究了不同电容（650μF，470μF,和220μF）的强脉冲光对液体食品模型模型中的食源性病原微生物铜绿假单胞菌，96孔细胞板，和聚碳酸酯膜模型在室温（25°C）和冷藏（4°C）环境下提供数据，以支持IPL灭菌装置在食品加工中的应用。
■IPL在浮游状态以及早期和成熟生物膜状态下对杀死铜绿假单胞菌非常有效，达到100%的目标杀伤率，99.99%,和94.33%对于给定数量的曝光。与浮游状态相比，在聚碳酸酯膜模型和96孔板模型中形成的生物膜对杀伤的抵抗力更强。达到同样的杀菌效果，闪烁次数随着电容的减小而增加。
■IPL对铜绿假单胞菌的杀菌效果受细菌状态的显着影响。电容越大,脉冲数越多,对铜绿假单胞菌的灭菌效果越好。
