背景:苍蝇被认为是通过机械手段传播的疾病的媒介,对人类健康构成重大风险。该研究旨在确定浦东新区苍蝇携带的肠病原体的患病率,以提供预防和控制苍蝇的策略。
方法:在2021年4月至11月之间,使用笼子诱捕技术从该地区的各个位置收集了样本,涵盖了各种栖息地,例如公园,住宅区,餐馆,和农贸市场。主要的苍蝇物种是使用冷冻显微和分类计数鉴定的,每管从不同的栖息地收集20个样本。使用GI_Trialv3TaqManTM微生物阵列筛选25种肠病原体。
结果:从6,400个位置中总共收集了3,875只苍蝇,导致每个笼子的平均苍蝇密度为0.61只。家蝇是最常见的物种,占39.85%,其次是sericataL.16.57%和peregrinaB.13.14%。189个样本中,93例肠病原体检测呈阳性,发现了九种不同的病原体。12.70%的样本都有寄生虫,比只有细菌或病毒的人更高的百分比。该研究发现,家畜M.sericata和Peregrina的肠道病原体少于L.sericata和B.peregrina,主要藏有人源芽孢杆菌,而不是细菌和病毒,如大肠杆菌,星状病毒,和Sapovirus.在弹簧测试期间,所有三种苍蝇都显示出肠病原体的检出率较低。在病原体种类最多的居民区发现了家畜。总共六个。相比之下,sericata和peregrina在农贸市场中发现了数量最多的病原体物种,总共六个和七个,分别。
结论:苍蝇有可能成为肠病原体传播的载体,从而对公众健康构成重大风险。
BACKGROUND: Flies are acknowledged as vectors of diseases transmitted through mechanical means and represent a significant risk to human health. The study aimed to determine the prevalence of enteropathogens carried by flies in Pudong New Area to inform strategies for preventing and controlling flies.
METHODS: Samples were collected from various locations in the area using cage trapping techniques between April and November 2021, encompassing various habitats such as parks, residential areas, restaurants, and farmers\' markets. The main fly species were identified using cryomicrography and taxonomic enumeration, with 20 samples per tube collected from different habitats. Twenty-five enteropathogens were screened using GI_Trial v3 TaqManTM microbial arrays.
RESULTS: A total of 3,875 flies were collected from 6,400 placements, resulting in an average fly density of 0.61 flies per cage. M. domestica were the most common species at 39.85%, followed by L. sericata at 16.57% and B. peregrina at 13.14%. Out of 189 samples, 93 tested positive for enteropathogens, with nine different pathogens being found. 12.70% of samples exclusively had
parasites, a higher percentage than those with only bacteria or viruses. The study found that M. domestica had fewer enteropathogens than L. sericata and B. peregrina, which primarily harbored B. hominis instead of bacteria and viruses such as E. coli, Astrovirus, and Sapovirus. During spring testing, all three fly species exhibited low rates of detecting enteropathogens. M. domestica were found in residential areas with the highest number of pathogen species, totaling six. In contrast, L. sericata and B. peregrina were identified in farmers\' markets with the highest number of pathogen species, totaling six and seven, respectively.
CONCLUSIONS: Flies have the potential to serve as vectors for the transmission of enteropathogens, thereby posing a substantial risk to public health.