口面肉芽肿病(OFG)是一种慢性炎症性疾病,其特征是口面组织的不嫩肿胀,其根本原因仍然未知。我们先前的研究表明,牙尖牙周炎(AP)参与了OFG的发展。为了表征OFG患者的AP细菌特征,并确定导致OFG的可能致病菌,使用16SrRNA基因测序对OFG患者和对照组的AP微生物组成进行比较.通过将细菌生长为菌落,然后纯化,建立了推定的细菌病原体的纯培养物。identification,和富集,然后注射到动物模型中以确定导致OFG的致病菌。显示了OFG患者中特定的AP微生物群特征,其特征是以厚壁门和变形杆菌为主,特别是链球菌属的成员,乳酸菌,还有奈瑟氏菌,被发现了。链球菌属。,干酪乳杆菌,亚黄奈瑟菌,细小静脉菌,和放线菌属。从OFG患者中分离并成功培养,然后注射到小鼠体内。最终,足垫注射N.subflava引起肉芽肿性炎症。IMPORTANCEInfectiousagentshavelongbeenconsideredtoplayaroleintheinitiationofOFG;however,微生物和OFG之间的直接因果关系尚未建立。在这项研究中,在OFG患者中发现了一种独特的AP微生物群特征.此外,我们成功地从OFG患者的AP病灶中分离出候选细菌,并评估其在实验室小鼠中的致病性.这项研究的结果可能有助于深入了解微生物在OFG发育中的作用。为OFG的靶向治疗方法提供依据。
Orofacial granulomatosis (OFG) is a chronic inflammatory disease characterized by nontender swelling of the orofacial tissues, the underlying cause of which remains unknown. Our previous study demonstrated that tooth apical periodontitis (AP) is involved in the development of OFG. To characterize the AP bacterial signatures of OFG patients and identify possible pathogenic bacteria that cause OFG, the compositions of the AP microbiotas in OFG patients and controls were compared using 16S rRNA gene sequencing. Pure cultures of putative bacterial pathogens were established by growing bacteria as colonies followed by purification, identification, and enrichment and then were injected into animal models to determine the causative bacteria contributing to OFG. A specific AP microbiota signature in the OFG patients was shown, characterized by the predominance of phyla Firmicutes and Proteobacteria, notably members of the genera Streptococcus, Lactobacillus, and
Neisseria, were found. Streptococcus spp., Lactobacillus casei,
Neisseria subflava, Veillonella parvula, and Actinomyces spp. from OFG patients were isolated and successfully cultured in vitro and then injected into mice. Ultimately, footpad injection with N. subflava elicited granulomatous inflammation. IMPORTANCE Infectious agents have long been considered to play a role in the initiation of OFG; however, a direct causal relationship between microbes and OFG has not yet been established. In this study, a unique AP microbiota signature was identified in OFG patients. Moreover, we successfully isolated candidate bacteria from AP lesions of OFG patients and assessed their pathogenicity in laboratory mice. Findings from this study may help provide in-depth insights into the role of microbes in OFG development, providing the basis for targeted therapeutic approaches for OFG.