三只猫,2至11岁,在安乐死或因呼吸窘迫而死亡后的3年内提交给明尼苏达大学兽医诊断实验室。胸片显示结节状,在所有情况下,整个肺野的软组织混浊。验尸时,大约60%至80%的肺实质通过多病灶扩大到合并,界限分明,米色,半坚固结节。组织学上,大量的中性粒细胞,更少的巨噬细胞,纤维蛋白,细胞和核仁碎片清除了肺实质。炎症灶包含革兰氏阴性球菌的聚集体。16srRNASanger测序和全基因组测序将在有氧条件下从所有猫的肺中分离出的细菌鉴定为新型奈瑟菌。基于全基因组序列分析,所有3个序列与密切相关的动物奈瑟菌NZLR134440T和动物奈瑟菌GCA002108605T共有92.71%和92.67%的平均核苷酸同一性,分别。与我们的分离株相比,使用DSM奈瑟氏菌21642和DSM21643菌株的计算机DNA-DNA杂交同一性分别为46.6%和33.8%。所有3个序列具有小于95%的平均核苷酸同一性和小于70%的DNA-DNA杂交同一性,表明这3个分离物是奈瑟球菌属的新种。奈瑟氏球菌感染。在放射学和病理学上类似于转移性肿瘤过程的猫中诱发栓塞性肺炎,在具有播散性的感染性肺部疾病的病例中,应在病因鉴别诊断中予以考虑,结节性肺模式。
Three cats, aged 2 to 11 years, presented to the University of Minnesota Veterinary Diagnostic Laboratory over a 3-year period following euthanasia or death due to respiratory distress. Thoracic radiographs revealed nodular, soft tissue opacities throughout the lung fields in all cases. On postmortem examination, approximately 60% to 80% of the lung parenchyma were expanded by multifocal to coalescing, well-demarcated, beige, semi-firm nodules. Histologically, large numbers of neutrophils, fewer macrophages, fibrin, and cellular and karyorrhectic debris effaced the pulmonary parenchyma. The inflammatory foci contained aggregates of gram-negative cocci. 16s rRNA Sanger sequencing and whole-genome sequencing identified the bacteria isolated from the lung of all cats under aerobic conditions as a novel Neisseria spp. Based on whole-genome sequence analysis, all 3 sequences shared 92.71% and 92.67% average nucleotide identity with closely related
Neisseria animaloris NZ LR134440T and
Neisseria animaloris GCA 002108605T, respectively. The in silico DNA-DNA hybridization identity compared to our isolates was 46.6% and 33.8% with strain DSM
Neisseria zoodegmatis 21642 and strain DSM 21643, respectively. All 3 sequences have less than 95% average nucleotide identity and less than 70% DNA-DNA hybridization identity, suggesting that the 3 isolates are a novel species of the genus
Neisseria. Infection with
Neisseria spp. induces an embolic pneumonia in cats that radiographically and pathologically resembles a metastatic neoplastic process and should be considered among the etiologic differential diagnoses in cases of infectious pulmonary disease with a disseminated, nodular lung pattern.