全球范围内,由于广泛的分散,种内多样性,和嗜盐生态系统的关键生态组成部分,嗜盐细菌被认为是生态的关键模型之一,适应性,和生物技术在盐碱环境中的应用研究。为了这个目标,本研究旨在启发植物生长促进特征,并研究嗜盐菌的系统基因组,卤化反硝化病毒ASH15,通过单分子实时(SMRT)测序技术。结果表明,菌株ASH15可以在高达25%(w/v)NaCl浓度的高盐度下存活,并表现出固氮等促进植物生长的性状,植物生长激素,和水解酶,维持盐胁迫。盆栽实验结果表明,在盐胁迫条件下,菌株ASH15显着增强了甘蔗植物的生长(根冠长度和重量)。此外,ASH15菌株基因组的测序分析表明,该菌株包含3,832,903bp的环状染色体,平均GC含量为37.54%:3721个预测的蛋白质编码序列(CDS),24个rRNA基因,和62个tRNA基因。基因组分析显示,与相容溶质(甘氨酸,甜菜碱,ectoine,羟基艾托宁,和谷氨酸)确认盐胁迫以及重金属抗性。此外,功能注释表明,菌株ASH15编码根定植的基因,生物膜的形成,植物激素IAA生产,固氮,磷酸盐代谢,和铁载体生产,有利于促进植物生长。菌株ASH15还具有对抗生素和病原体的基因抗性。此外,分析还显示,基因组菌株ASH15具有插入序列和CRISPR,这表明它有能力通过水平基因转移获得新基因,并获得对病毒攻击的免疫力。这项工作提供了有关V.halodenitrificansASH15耐受盐胁迫的机制的知识。深度基因组分析,鉴定了参与类异戊二烯生物合成的MVA途径,更准确地说,“角鲨烯。\"角鲨烯有各种应用,比如抗氧化剂,抗癌剂,抗老化剂,血液预防剂,抗菌剂,疫苗和药物载体的佐剂,和解毒剂。我们的研究结果表明,菌株ASH15在农业等行业具有巨大的潜力,制药,化妆品,和食物。
Globally, due to widespread dispersion, intraspecific diversity, and crucial ecological components of halophilic ecosystems, halophilic bacteria is considered one of the key models for ecological, adaptative, and biotechnological applications research in saline environments. With this aim, the present study was to enlighten the plant growth-promoting features and investigate the systematic genome of a halophilic bacteria, Virgibacillus halodenitrificans ASH15, through single-molecule real-time (SMRT) sequencing technology. Results showed that strain ASH15 could survive in high salinity up to 25% (w/v) NaCl concentration and express plant growth-promoting traits such as nitrogen fixation, plant growth hormones, and hydrolytic enzymes, which sustain salt stress. The results of pot experiment revealed that strain ASH15 significantly enhanced sugarcane plant growth (root shoot length and weight) under salt stress conditions. Moreover, the sequencing analysis of the strain ASH15 genome exhibited that this strain contained a circular chromosome of 3,832,903 bp with an average G+C content of 37.54%: 3721 predicted protein-coding sequences (CDSs), 24 rRNA genes, and 62 tRNA genes. Genome analysis revealed that the genes related to the synthesis and transport of compatible solutes (glycine, betaine, ectoine, hydroxyectoine, and glutamate) confirm salt stress as well as heavy metal resistance. Furthermore, functional annotation showed that the strain ASH15 encodes genes for root colonization, biofilm formation, phytohormone IAA production, nitrogen fixation, phosphate metabolism, and siderophore production, which are beneficial for plant growth promotion. Strain ASH15 also has a gene resistance to antibiotics and pathogens. In addition, analysis also revealed that the genome strain ASH15 has insertion sequences and CRISPRs, which suggest its ability to acquire new genes through horizontal gene transfer and acquire immunity to the attack of viruses. This work provides knowledge of the mechanism through which V. halodenitrificans ASH15 tolerates salt stress. Deep genome analysis, identified MVA pathway involved in biosynthesis of
isoprenoids, more precisely \"Squalene.\" Squalene has various applications, such as an antioxidant, anti-cancer agent, anti-aging agent, hemopreventive agent, anti-bacterial agent, adjuvant for vaccines and drug carriers, and detoxifier. Our findings indicated that strain ASH15 has enormous potential in industries such as in agriculture, pharmaceuticals, cosmetics, and food.