目的:牙周炎是一种慢性感染性疾病,导致牙周支持组织的炎症破坏。白细胞介素14(IL-14),白细胞介素16(IL-16)和血清淀粉样蛋白A(SAA)已被证明在炎症性疾病中异常表达。因此,本研究旨在分析IL-14、1L-16和SAA在牙周炎中的表达及其潜在临床价值。
方法:共招募100例牙周炎患者和100例健康志愿者,收集唾液和血清样本。然后是C反应蛋白(CRP),降钙素原(PCT),采用Elisa试剂盒检测牙周炎患者唾液和血清中IL-14、IL-16和SAA水平。此外,CRP的意义,PCT,采用受试者工作特征(ROC)分析牙周炎患者的IL-14,1L-16和SAA。
结果:结果显示,PCT,牙周炎患者唾液和血清中IL-14、IL-16和SAA水平显著升高。此外,唾液CRP曲线下面积(AUC),PCT,诊断牙周炎的IL-14、IL-16和SAA分别为0.9035、0.9435、0.9508、0.9500和0.9467。血清CRP的AUC,PCT,诊断牙周炎的IL-14、IL-16和SAA分别为0.9035、0.9435、0.9508、0.9500和0.9467。更重要的是,IL-14、IL-16和SAA联合CRP和PCT的诊断价值提高。
结论:本研究表明,IL-14、IL-16和SAA在牙周炎患者中的表达上调,对牙周炎的诊断具有重要意义。
OBJECTIVE: Periodontitis is a chronic infectious disease, which leads to inflammatory destruction of periodontal supporting tissues. Interleukin 14 (IL-14), Interleukin 16 (IL-16) and serum amyloid A (SAA) have been demonstrated to be abnormally expressed in inflammatory diseases. Therefore, this study was performed to analyzed the expression and potential clinical values of IL-14, 1L-16 and SAA in periodontitis.
METHODS: A total of 100 periodontitis patients and 100 healthy volunteers were recruited and the saliva and serum samples were collected. Then the C-reactive protein (CRP), procalcitonin (PCT), IL-14, 1L-16 and SAA levels in the saliva and serum of periodontitis patients were measured by Elisa kits. Besides, the significance of CRP, PCT, IL-14, 1L-16 and SAA in periodontitis patients were analyzed by receiver operating characteristic (ROC) analysis.
RESULTS: The results showed that CRP, PCT, IL-14, 1L-16 and SAA levels were significantly increased in the the saliva and serum of the periodontitis patients. Additionally, the area under the curve (AUC) of saliva CRP, PCT, IL-14, 1L-16 and SAA for the diagnosis of periodontitis were 0.9035, 0.9435, 0.9508, 0.9500 and 0.9467, respectively. The AUC of serum CRP, PCT, IL-14, 1L-16 and SAA for the diagnosis of periodontitis were 0.9035, 0.9435, 0.9508, 0.9500 and 0.9467, respectively. What\'s more, the diagnostic value of IL-14, 1L-16 and SAA were enhanced when combining with CRP and PCT.
CONCLUSIONS: This study demonstrated that IL-14, IL-16 and SAA expressions were upregulated in periodontitis patients and exhibited a significant significance for periodontitis diagnosis.