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Abstract：
OBJECTIVE: Elevated serum interleukin-16 (IL-16) levels have been reported in gastric cancer (GC) tissues; however, the role of IL-16 genotypes in GC susceptibility remains largely unexplored. This study aimed to investigate the contribution of IL-16 genotypes to GC susceptibility and to assess their interactions with smoking, alcohol drinking, and Helicobacter pylori (H. pylori) infection.
METHODS: Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) methodology was employed to determine IL-16 rs4778889, rs11556218, and rs4072111 genotypic characteristics in 161 patients with GC and 483 controls.
RESULTS: Significant differences were observed in the distribution of genotypic (p=0.0009) and allelic (p=0.0002) frequencies of IL-16 rs11556218 among cases and controls. Specifically, the frequencies of TG and GG genotypes of IL-16 rs11556218 were 37.3% and 6.8% among patients with GC, respectively, which were higher than those among the controls (26.7% and 2.7%). In contrast, no significant differences were found concerning IL-16 rs4778889 or rs4072111. Notably, individuals with IL-16 rs11556218 TT genotypes exhibited significant protective effects against GC when exposed to risk factors, such as smoking, alcohol drinking, and H. pylori infection.
CONCLUSIONS: IL-16 rs11556218 T allele was associated with reduced susceptibility to GC. Furthermore, carriers of the TT genotype showed protection against GC risk factors, including smoking, alcohol drinking, and H. pylori infection. These findings provide valuable insights into the potential role of IL-16 genotypes in GC development and their interactions with lifestyle and infectious factors.
METHODS: Polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) methodology was employed to determine IL-16 rs4778889, rs11556218, and rs4072111 genotypic characteristics in 161 patients with GC and 483 controls.
RESULTS: Significant differences were observed in the distribution of genotypic (p=0.0009) and allelic (p=0.0002) frequencies of IL-16 rs11556218 among cases and controls. Specifically, the frequencies of TG and GG genotypes of IL-16 rs11556218 were 37.3% and 6.8% among patients with GC, respectively, which were higher than those among the controls (26.7% and 2.7%). In contrast, no significant differences were found concerning IL-16 rs4778889 or rs4072111. Notably, individuals with IL-16 rs11556218 TT genotypes exhibited significant protective effects against GC when exposed to risk factors, such as smoking, alcohol drinking, and H. pylori infection.
CONCLUSIONS: IL-16 rs11556218 T allele was associated with reduced susceptibility to GC. Furthermore, carriers of the TT genotype showed protection against GC risk factors, including smoking, alcohol drinking, and H. pylori infection. These findings provide valuable insights into the potential role of IL-16 genotypes in GC development and their interactions with lifestyle and infectious factors.
摘要：
目的：据报道，胃癌（GC）组织中血清白细胞介素-16（IL-16）水平升高；然而，IL-16基因型在GC易感性中的作用在很大程度上仍未被探索.本研究旨在调查IL-16基因型对GC易感性的贡献，并评估其与吸烟的相互作用。饮酒,和幽门螺杆菌(H.幽门螺杆菌)感染。
方法：采用基于聚合酶链反应的限制性片段长度多态性（PCR-RFLP）方法确定161例GC患者和483例对照患者的IL-16rs4778889，rs11556218和rs4072111基因型特征。
结果：在病例和对照组之间，IL-16rs11556218的基因型（p=0.0009）和等位基因（p=0.0002）频率的分布存在显着差异。具体来说,在GC患者中，IL-16rs11556218的TG和GG基因型频率分别为37.3%和6.8%，分别,高于对照组(26.7%和2.7%)。相比之下,关于IL-16rs4778889或rs4072111，没有发现显着差异。值得注意的是,具有IL-16rs11556218TT基因型的个体在暴露于危险因素时表现出对GC的显着保护作用，比如吸烟，饮酒,和幽门螺杆菌感染。
结论：IL-16rs11556218T等位基因与GC易感性降低相关。此外，TT基因型的携带者对GC危险因素有保护作用，包括吸烟,饮酒,和幽门螺杆菌感染。这些发现为IL-16基因型在GC发育中的潜在作用及其与生活方式和感染因素的相互作用提供了有价值的见解。
方法：采用基于聚合酶链反应的限制性片段长度多态性（PCR-RFLP）方法确定161例GC患者和483例对照患者的IL-16rs4778889，rs11556218和rs4072111基因型特征。
结果：在病例和对照组之间，IL-16rs11556218的基因型（p=0.0009）和等位基因（p=0.0002）频率的分布存在显着差异。具体来说,在GC患者中，IL-16rs11556218的TG和GG基因型频率分别为37.3%和6.8%，分别,高于对照组(26.7%和2.7%)。相比之下,关于IL-16rs4778889或rs4072111，没有发现显着差异。值得注意的是,具有IL-16rs11556218TT基因型的个体在暴露于危险因素时表现出对GC的显着保护作用，比如吸烟，饮酒,和幽门螺杆菌感染。
结论：IL-16rs11556218T等位基因与GC易感性降低相关。此外，TT基因型的携带者对GC危险因素有保护作用，包括吸烟,饮酒,和幽门螺杆菌感染。这些发现为IL-16基因型在GC发育中的潜在作用及其与生活方式和感染因素的相互作用提供了有价值的见解。
