姜黄素(Cur)是一种具有各种有益特性的植物化学物质,包括抗氧化剂,抗炎,和抗癌活动。然而,它的疏水性,生物利用度差,和稳定性限制了其在许多生物学方法中的应用。在这项研究中,合成了一种新型两亲性壳聚糖壁材。该过程是通过以琥珀酸酐(SA)作为亲水基团和脱氧胆酸(DA)作为疏水基团接枝壳聚糖进行的;1H-NMR,FTIR,采用XRD对两亲性壳聚糖(CS-SA-DA)进行表征。使用低成本,基于无机溶剂的程序,CS-SA-DA自组装以负载Cur纳米胶束。这种两亲性聚合物形成了具有核壳结构的自组装胶束,临界胶束浓度(CMC)为0.093mg·mL-1。通过自组装制备负载Cur的纳米胶束,并通过纳米粒度电位分析仪和透射电子显微镜(TEM)进行表征。球形负载Cur的胶束的平均粒度为770nm。药物包封率和载药量分别达到80.80±0.99%和19.02±0.46%,分别。姜黄素从胶束中的体外释放曲线显示出活性药物分子的恒定释放。斑马鱼的细胞毒性研究和毒性测试显示了该递送系统的相当功效和安全性。此外,结果表明,姜黄素在胶束中的包封提高了其稳定性,抗氧化剂,和抗炎活性。
Curcumin (Cur) is a phytochemical with various beneficial properties, including antioxidant, anti-inflammatory, and anticancer activities. However, its hydrophobicity, poor bioavailability, and stability limit its application in many biological approaches. In this study, a novel amphiphilic chitosan wall material was synthesized. The process was carried out via grafting chitosan with succinic anhydride (SA) as a hydrophilic group and deoxycholic acid (DA) as a hydrophobic group; 1H-NMR, FTIR, and XRD were employed to characterize the amphiphilic chitosan (CS-SA-DA). Using a low-cost, inorganic solvent-based procedure, CS-SA-DA was self-assembled to load Cur nanomicelles. This amphiphilic polymer formed self-assembled micelles with a core-shell structure and a critical micelle concentration (CMC) of 0.093 mg·mL-1. Cur-loaded nanomicelles were prepared by self-assembly and characterized by the Nano Particle Size Potential Analyzer and transmission electron microscopy (TEM). The mean particle size of the spherical Cur-loaded micelles was 770 nm. The drug entrapment efficiency and loading capacities were up to 80.80 ± 0.99% and 19.02 ± 0.46%, respectively. The in vitro release profiles of curcumin from micelles showed a constant release of the active drug molecule. Cytotoxicity studies and toxicity tests for zebrafish exhibited the comparable efficacy and safety of this delivery system. Moreover, the results showed that the entrapment of curcumin in micelles improves its stability, antioxidant, and anti-inflammatory activity.