通过研究没食子酸对视网膜神经节细胞氧化应激的抑制作用,评价没食子酸对视神经的保护作用。100只雄性SD大鼠随机分为4组:正常对照组,单纯高眼压组,0.5%没食子酸实验组,1%没食子酸实验组。HE染色,免疫荧光,DHE染色,蛋白质印迹,采用q-PCR方法观察没食子酸对急性高眼压大鼠视网膜的抗氧化作用。SD大鼠视网膜HE染色证实RGCs细胞核清晰,正常对照组的RNFL厚度是规则的,单纯高眼压(IOP)组和没食子酸组的RGC核破裂和溶解,RNFL的厚度明显增厚,与单纯高眼压组相比,没食子酸组RNFL厚度显著降低(p<0.05)。DHE染色显示单纯高眼压组ROS含量较正常对照组显著升高,施用没食子酸后ROS含量明显下降(p<0.05)。用Brn-3a抗体免疫荧光染色证实,与正常对照组相比,单纯高眼压组的RGCs数量明显减少,而在施用没食子酸后,没食子酸组RGC数量明显多于单纯高眼压组(p<0.05)。WesternBlot和q-PCR证实单纯高眼压组视网膜组织中缺氧诱导因子1α(HIF-1α)蛋白含量和转录水平显著升高,没食子酸能抑制HIF-1α蛋白含量(p<0.05),降低转录因子水平(p<0.05)。没食子酸通过抑制急性眼压升高大鼠的氧化应激对RGC具有保护作用。
To evaluate the protective effect of gallic acid on the optic nerve by studying the inhibitory effect of gallic acid on oxidative stress in retinal ganglion cells. 100 male SD rats were randomly divided into four groups: normal control group, simple high IOP group, 0.5% gallic acid experimental group, and 1% gallic acid experimental group. HE staining, immunofluorescence, DHE staining, Western blot, and q-PCR were used to observe the antioxidant effect of gallic acid on the retina of acute ocular hypertension rats. HE staining of the retina of SD rats confirmed that the nucleus of RGCs was clear, the thickness of the RNFL was regular in the normal control group, and the nucleus of RGCs was ruptured and lysed in the simple high intraocular pressure (IOP) group and the gallic acid group, and the thickness of the RNFL was significantly thickened, but the thickness of the RNFL in the gallic acid group was significantly reduced compared with that in the simple high IOP group (p < 0.05). DHE staining showed that ROS content in the simple high IOP group was significantly increased compared with the normal control group, and ROS content was significantly decreased after the application of gallic acid (p < 0.05). Immunofluorescence staining with Brn-3a antibody confirmed that the number of RGCs was significantly reduced in the simple high IOP group compared with the normal control group, whereas after application of gallic acid, the number of RGCs was significantly more in the gallic acid group than in the simple high IOP group (p < 0.05). Western Blot and q-PCR confirmed that hypoxia-inducing factor 1α (HIF-1α) protein content and transcription level were significantly increased in the retinal tissue of the simple high IOP group, and gallic acid could inhibit HIF-1α protein content (p < 0.05) and reduce transcription factor level (p < 0.05). Gallic acid exerts a protective effect on RGC by inhibiting oxidative stress in rats with acute IOP elevation.